HRP Anti-Aldolase antibody

• WB

Supplier Data

Western blot - HRP Anti-Aldolase antibody (AB181662)

4-12% tris glycine gradient gel for SDS-PAGE

All lanes:

Western blot - HRP Anti-Aldolase antibody (ab181662) at 1/1000 dilution

All lanes:

A293 whole cell lysate

Predicted band size: 39 kDa

true

• IP

Supplier Data

Immunoprecipitation - HRP Anti-Aldolase antibody (AB181662)

Immunoprecipitation was performed with 300 μl of anti-Aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS. Rabbit Adolase (from rabbit muscle) was used.

1. Purified Aldolase (1μg)

2. Precipitation negative control (antiserum + No antigen)

3. 300 μl antiserum + 20 μl Aldolase

4. 300 μl antiserum + 80 μl Aldolase

5. 300 μl antiserum + 120 μl Aldolase

6. 300 μl antiserum + 150 μl Aldolase

7. 300 μl antiserum + 300 μl Aldolase

All lanes:

Immunoprecipitation - HRP Anti-Aldolase antibody (ab181662)

Predicted band size: 39 kDa

false

• IP

Supplier Data

Immunoprecipitation - HRP Anti-Aldolase antibody (AB181662)

Immunoprecipitation and Western Blot analysis.

300 μl aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are :

1. Purified aldolase

2. 300 μl antiserum with no antigen (negative control)

3. 300 μl antiserum with ~100 μl aldolase (2.5 mg/ml)

4. 300 μl antiserum with ~200 μl aldolase (2.5 mg/ml)

All lanes:

Immunoprecipitation - HRP Anti-Aldolase antibody (ab181662)

Predicted band size: 39 kDa

false