Goat Polyclonal Aldolase antibody - conjugated to HRP. Suitable for IP, WB and reacts with Rabbit, Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rabbit ALDOA.
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- Oncology reports 46:2021Propofol mediates pancreatic cancer cell activity through the repression of ADAM8 via SP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYutong Gao,Yu Zhou,Chunlin Wang,Klarke M Sample,Xiangdi Yu,Yaacov Ben-DavidPubMed 34617574
- Bioscience trends 15:100-1062021MCM4 in human hepatocellular carcinoma: a potent prognostic factor associated with cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Xu,Xueling Yang,Tongguo Si,Haipeng Yu,Yong Li,Wenge Xing,Zhi GuoPubMed 33716256
- Molecular medicine reports 22:1727-17362020Effects of GPR81 silencing combined with cisplatin stimulation on biological function in hypopharyngeal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiaojing Jia et. al.PubMed 32582969
- Molecular medicine reports 20:4654-46642019Exploring the active mechanism of berberine against HCC by systematic pharmacology and experimental validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Song et. al.PubMed 31545468
- Medical science monitor : international medical jo 25:4583-45892019Effects of Vitamin A on Expressions of Apoptosis Genes Bax and Bcl-2 in Epithelial Cells of Corneal Tissues Induced by Benzalkonium Chloride in Mice with Dry Eye.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeihua Zhang et. al.PubMed 31257361
Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- HRP
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.27% Tripotassium orthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Rabbit ALDOA. Database link P00883
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Rabbit | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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5 products for Alternative Product
Target data
Function
Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, may also function as scaffolding protein (By similarity).
Alternative names
ALDA, ALDOA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase
Recommended products
Goat Polyclonal Aldolase antibody - conjugated to HRP. Suitable for IP, WB and reacts with Rabbit, Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rabbit ALDOA.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- HRP
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.27% Tripotassium orthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Rabbit ALDOA. Database link P00883
- Purity
- IgG fraction
- Concentration
- Purification notes
IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldolase also known as fructose-bisphosphate aldolase is an enzyme that plays a critical role in glycolysis catalyzing the reversible cleavage of fructose 16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Aldolase is a homotetramer with a molecular mass of approximately 158 kDa. It is highly expressed in liver muscle and brain tissues. Aldolase consists of three isoforms: aldolase A B and C each predominant in different tissues contributing to tissue-specific roles within the organism.
- Biological function summary
Aldolase catalyzes an important reaction in energy metabolism ensuring the continuation of glycolytic flux which is essential for ATP production. Aldolases do not form part of larger protein complexes. However they interact with other enzymes within the glycolytic pathway to facilitate a smooth metabolic flow. Moreover aldolase A plays an additional role in gluconeogenesis the pathway involved in synthesizing glucose from non-carbohydrate sources.
- Pathways
Aldolase is an essential part of glycolysis and gluconeogenesis pathways. In glycolysis it partners with enzymes like phosphofructokinase and enolase to breakdown glucose for energy production. During gluconeogenesis aldolase works with enzymes including fructose-16-bisphosphatase to create glucose vital for maintaining blood sugar levels during fasting. Through these pathways aldolase ensures balance between energy-producing and energy-consuming states within the cell.
- Associated diseases and disorders
Mutations or deficiencies in aldolase particularly aldolase A have been linked to glycogen storage disease type XII which results in muscle weakness and exercise intolerance. Aldolase B deficiency is known to cause hereditary fructose intolerance leading to liver and renal complications. These conditions highlight the importance of aldolase in maintaining metabolic health with implications for proteins within the same pathways potentially affecting their functions and expression levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - HRP Anti-Aldolase antibody (ab181662)
4-12% tris glycine gradient gel for SDS-PAGE
All lanes: Western blot - HRP Anti-Aldolase antibody (ab181662) at 1/1000 dilution
All lanes: A293 whole cell lysate
Developed using the ECL technique.
Predicted band size: 39 kDa
Immunoprecipitation - HRP Anti-Aldolase antibody (ab181662)
Immunoprecipitation and Western Blot analysis.
300 μl aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are:
1. Purified aldolase
2. 300 μl antiserum with no antigen (negative control)
3. 300 μl antiserum with ~100 μl aldolase (2.5 mg/ml)
4. 300 μl antiserum with ~200 μl aldolase (2.5 mg/ml)
All lanes: Immunoprecipitation - HRP Anti-Aldolase antibody (ab181662)
Predicted band size: 39 kDa
Immunoprecipitation - HRP Anti-Aldolase antibody (ab181662)
Immunoprecipitation was performed with 300 μl of anti-Aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS. Rabbit Adolase (from rabbit muscle) was used.
1. Purified Aldolase (1μg)
2. Precipitation negative control (antiserum + No antigen)
3. 300 μl antiserum + 20 μl Aldolase
4. 300 μl antiserum + 80 μl Aldolase
5. 300 μl antiserum + 120 μl Aldolase
6. 300 μl antiserum + 150 μl Aldolase
7. 300 μl antiserum + 300 μl Aldolase
All lanes: Immunoprecipitation - HRP Anti-Aldolase antibody (ab181662)
Predicted band size: 39 kDa
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