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AB305831

HRP Anti-Aldolase B antibody [EPR3138Y]

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Rabbit Recombinant Monoclonal Aldolase B antibody - conjugated to HRP.

View Alternative Names

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

  • 578 PE

    PE Anti-Aldolase B antibody [EPR3138Y]

  • 660 APC

    APC Anti-Aldolase B antibody [EPR3138Y]

  • Unconjugated

    Anti-Aldolase B + Aldolase C antibody [EPR3138Y]

  • Carrier free

    Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3138Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase B and Aldolase C are enzymes playing key roles in breaking down fructose and glucose in glycolysis and gluconeogenesis. Aldolase B also known as fructose-bisphosphate aldolase B has a mass of approximately 39 kDa and is mainly found in the liver kidney and small intestine. Aldolase C with a similar mass to Aldolase B is primarily expressed in the brain and nervous tissues. These enzymes are part of the aldolase family which catalyzes the conversion of fructose-16-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate.
Biological function summary

Both Aldolase B and C participate in key metabolic processes. Aldolase B is essential for fructose metabolism and degradation being especially important in tissues where fructose is a main sugar source. It is not part of a larger protein complex. Aldolase C on the other hand is significant in energy production in neurons contributing to overall brain metabolism. Both enzymes contribute to the conversion of sugars into energy necessary for cellular functions.

Pathways

Aldolase enzymes are integral to the glycolysis and gluconeogenesis pathways. In glycolysis they participate in the step where fructose-16-bisphosphate splits into two trioses facilitating energy release from glucose. In gluconeogenesis the reverse occurs assisting in the production of glucose. These pathways are important for maintaining energy balance in cells. Aldolase B notably interacts with fructokinase which phosphorylates fructose to fructose-1-phosphate the substrate for aldolase action.

Deficiencies or mutations in Aldolase B are linked to hereditary fructose intolerance (HFI) a disorder where fructose ingestion leads to toxic accumulation within cells and severe symptoms. Both Aldolase B and C can also be markers in conditions like hepatocellular carcinoma and neurodegenerative disorders. In HFI the deficiency in Aldolase B function leads to accumulations affecting liver kidney and digestive tissues while disturbances in Aldolase C may impact neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed : 10970798, PubMed : 12205126, PubMed : 20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed : 35122041).
See full target information ALDOB
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