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Rabbit Recombinant Monoclonal alpha Sarcoglycan antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780), expandable thumbnail
  • Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

Alternative names

ADL, DAG2, SGCA, Alpha-sarcoglycan, Alpha-SG, 50 kDa dystrophin-associated glycoprotein, Adhalin, Dystroglycan-2, 50DAG

Rabbit Recombinant Monoclonal alpha Sarcoglycan antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14773
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha Sarcoglycan also known as Adhalin or 50DAG is a protein with a molecular mass of approximately 50 kDa. It is an important component in skeletal and cardiac muscle tissue. This protein is part of the dystrophin-glycoprotein complex (DGC) which connects the cytoskeleton of a muscle fiber to the extracellular matrix. Alpha Sarcoglycan is primarily expressed in striated muscle cells including those of skeletal and cardiac muscles but also at low levels in other tissues.

Biological function summary

Alpha Sarcoglycan contributes to the integrity and function of muscle fibers by stabilizing the DGC which includes sarcoglycans and other associated proteins. This stabilization provides mechanical support during muscle contraction and stretching. Alpha Sarcoglycan's role in the complex ensures proper signal transduction and protects muscle fibers from injury that may occur through mechanical stress. The protein's interaction with other sarcoglycans such as beta gamma and delta facilitates these structural and signaling functions.

Pathways

Alpha Sarcoglycan is involved in muscle development and maintenance pathways. It plays a role in the mechanotransduction signaling pathway which helps cells convert mechanical stimuli into chemical activity. The DGC including alpha Sarcoglycan works closely with dystrophin an important protein in the process. This relationship is essential for maintaining the structural integrity of muscle fibers and impacts pathways regulating muscle repair and regeneration.

Associated diseases and disorders

Mutations in the alpha Sarcoglycan gene are linked to limb-girdle muscular dystrophy type 2D (LGMD2D). This condition is characterized by progressive muscle weakness particularly affecting the shoulders and hips. The disruption of alpha Sarcoglycan affects its interaction with other sarcoglycan proteins leading to the disassembly of the DGC and causing increased muscle cell damage. Understanding the role of alpha Sarcoglycan can also provide insights into other disorders related to defects in the DGC.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780), expandable thumbnail

    Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780)

    Alpha Sarcoglycan Western blot staining using rabbit Anti-alpha Sarcoglycan antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780) at 1/1000 dilution

    Lane 1: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 2: Rat skeletal muscle tissue lysate at 20 µg

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 6s

  • Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780), expandable thumbnail

    Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780)

    Alpha Sarcoglycan Western blot staining using rabbit Anti-alpha Sarcoglycan antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: cerebellum

    The identity of the lower MW band around 37 kDa is supposed to be non-specific binding.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780) at 1/1000 dilution

    Lane 1: Human skeletal muscle tissue lysate at 20 µg

    Lane 2: Human cerebellum tissue lysate at 20 µg

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780)

    Alpha Sarcoglycan Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cardiac muscle using rabbit Anti-alpha Sarcoglycan antibody

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling alpha Sarcoglycan with ab323780 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on human cardiac muscle.

    The section was incubated with ab323780 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780)

    Alpha Sarcoglycan Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human skeletal muscle using rabbit Anti-alpha Sarcoglycan antibody

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab323780 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on human skeletal muscle.

    The section was incubated with ab323780 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780)

    Alpha Sarcoglycan Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-alpha Sarcoglycan antibody

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling alpha Sarcoglycan with ab323780 at 1/100 (5.0 ug/ml) dilution.

    Negative control: no staining on human cerebrum.

    The section was incubated with ab323780 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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