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AB323780

HRP Anti-alpha Sarcoglycan antibody [EPR14773]

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Rabbit Recombinant Monoclonal alpha Sarcoglycan antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

ADL, DAG2, SGCA, Alpha-sarcoglycan, Alpha-SG, 50 kDa dystrophin-associated glycoprotein, Adhalin, Dystroglycan-2, 50DAG

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling alpha Sarcoglycan with ab323780 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on human cerebrum.
The section was incubated with ab323780 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab323780 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human skeletal muscle.
The section was incubated with ab323780 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling alpha Sarcoglycan with ab323780 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human cardiac muscle.
The section was incubated with ab323780 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)
  • WB

Supplier Data

Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : cerebellum

The identity of the lower MW band around 37 kDa is supposed to be non-specific binding.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Observed band size: 50 kDa,36 kDa

false

Exposure time: 3s

Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)
  • WB

Supplier Data

Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (AB323780)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - HRP Anti-alpha Sarcoglycan antibody [EPR14773] (ab323780) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Observed band size: 50 kDa,36 kDa

false

Exposure time: 6s

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-alpha Sarcoglycan antibody [EPR14773]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-alpha Sarcoglycan antibody [EPR14773]

  • Unconjugated

    Anti-alpha Sarcoglycan antibody [EPR14773]

  • Carrier free

    Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14773

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Mouse, Rat, Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Sarcoglycan also known as Adhalin or 50DAG is a protein with a molecular mass of approximately 50 kDa. It is an important component in skeletal and cardiac muscle tissue. This protein is part of the dystrophin-glycoprotein complex (DGC) which connects the cytoskeleton of a muscle fiber to the extracellular matrix. Alpha Sarcoglycan is primarily expressed in striated muscle cells including those of skeletal and cardiac muscles but also at low levels in other tissues.
Biological function summary

Alpha Sarcoglycan contributes to the integrity and function of muscle fibers by stabilizing the DGC which includes sarcoglycans and other associated proteins. This stabilization provides mechanical support during muscle contraction and stretching. Alpha Sarcoglycan's role in the complex ensures proper signal transduction and protects muscle fibers from injury that may occur through mechanical stress. The protein's interaction with other sarcoglycans such as beta gamma and delta facilitates these structural and signaling functions.

Pathways

Alpha Sarcoglycan is involved in muscle development and maintenance pathways. It plays a role in the mechanotransduction signaling pathway which helps cells convert mechanical stimuli into chemical activity. The DGC including alpha Sarcoglycan works closely with dystrophin an important protein in the process. This relationship is essential for maintaining the structural integrity of muscle fibers and impacts pathways regulating muscle repair and regeneration.

Mutations in the alpha Sarcoglycan gene are linked to limb-girdle muscular dystrophy type 2D (LGMD2D). This condition is characterized by progressive muscle weakness particularly affecting the shoulders and hips. The disruption of alpha Sarcoglycan affects its interaction with other sarcoglycan proteins leading to the disassembly of the DGC and causing increased muscle cell damage. Understanding the role of alpha Sarcoglycan can also provide insights into other disorders related to defects in the DGC.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
See full target information SGCA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com