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Rabbit Recombinant Monoclonal APG5L/ATG5 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB199560), expandable thumbnail
  • Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB199560), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Rat
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed:19666601).

Alternative names

APG5L, ASP, ATG5, Autophagy protein 5, APG5-like, Apoptosis-specific protein

Recommended products

Rabbit Recombinant Monoclonal APG5L/ATG5 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1755(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

APG5L commonly known as ATG5 or ATG5 protein plays a significant role in autophagy. This molecular component has a molecular weight of approximately 32 kDa. ATG5 is expressed in multiple tissue types with a higher expression profile in immune-related cells. The protein is essential for the formation of the autophagosome as it conjugates with ATG12 to form a complex that is critical for autophagosome membrane elongation.

Biological function summary

ATG5 plays an important part in the cellular process of autophagy affecting the degradation and recycling of cellular components. It is a part of a complex with ATG12 and ATG16L1 which facilitates the membrane building of the autophagosome. This protein is important for maintaining cellular homeostasis allowing the removal of damaged proteins and organelles and is involved in responses to stress and starvation.

Pathways

ATG5 is an important component in the autophagy pathway and is involved in cellular maintenance processes. This pathway is vital for the regulation of cell growth and metabolism. ATG5 works closely with other autophagy-related proteins such as LC3. The ATG5-ATG12 complex helps in the lipidation of LC3 which is critical for autophagosome maturation connecting its function to the mTOR signaling pathway that regulates many cellular processes.

Associated diseases and disorders

Disruption in ATG5 function is linked to neurodegenerative diseases and cancer. For instance dysregulation of autophagy due to ATG5 dysfunction may result in the progression of neurodegenerative disorders like Alzheimer's disease. Additionally in certain cancers altered autophagy can influence the survival of cancer cells. The interplay between ATG5 and other proteins like p53 and Bcl-2 is under investigation to understand its role in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab199560), expandable thumbnail

    Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab199560)

    ab199560 was shown to specifically react with ATG5 in wild-type HAP1 cells as signal was lost in ATG5 knockout cells. Wild-type and ATG5 knockout samples were subjected to SDS-PAGE. ab199560 was incubated overnight at 4°C at a 1/5000 dilution.

    All lanes: Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab199560) at 1/5000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: APG5L/ATG5 knockout HAP1 cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa

    Observed band size: 52 kDa

    Exposure time: 8min

  • Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab199560), expandable thumbnail

    Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab199560)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199560 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab199560) at 1/5000 dilution

    Lane 1: Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

    Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa

    Observed band size: 50 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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