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AB27622

HRP Anti-Apolipoprotein B antibody

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(3 Publications)

Anti-Apolipoprotein B antibody - HRP conjugated (ab27622) is a goat polyclonal antibody detecting Apolipoprotein B in Western Blot, ELISA. Suitable for Human.

- Trusted since 2006

View Alternative Names

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

ELISA, WB

applications

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Reactivity data

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Product details

What is this antibody validated in?
HRP Anti-Apolipoprotein B antibody (ab27622) is a goat polyclonal antibody and is validated for use in Western Blot (WB), ELISA in Human samples.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by human apo B-100-Sepharose™ affinity column.
Storage buffer
pH: 6.5 - 7.4 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1% BSA, 0.58% Sodium chloride, 0.134% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.
Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
See full target information APOB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 61:455-463 PubMed31888979

2020

Separation of postprandial lipoproteins: improved purification of chylomicrons using an ApoB100 immunoaffinity method.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Marie Jones,Russell Caccavello,Sergiu P Palii,Clive R Pullinger,John P Kane,Kathleen Mulligan,Alejandro Gugliucci,Jean-Marc Schwarz

Redox report : communications in free radical rese 19:154-60 PubMed24524596

2014

Evidence for the presence of active paraoxonase 1 in small-dense low-density lipoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Gugliucci,Russell Caccavello,Kazuhiko Kotani,Satoshi Kimura

Clinical biochemistry 47:475-7 PubMed24362269

2013

Small-dense low-density lipoproteins are the predominant apoB-100-containing lipoproteins in cord blood.

Applications

WB

Species

Human

Alejandro Gugliucci,Masahide Numaguchi,Russell Caccavello,Satoshi Kimura
View all publications
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Product promise

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