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AB209317

HRP Anti-ASH2L antibody [EPR13106]

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(1 Publication)

Rabbit Recombinant Monoclonal ASH2L antibody - conjugated to HRP. Suitable for WB and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

ASH2L1, ASH2L, Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein

1 Images
Western blot - HRP Anti-ASH2L antibody [EPR13106] (AB209317)
  • WB

Lab

Western blot - HRP Anti-ASH2L antibody [EPR13106] (AB209317)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209317 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ASH2L antibody [EPR13106] (ab209317) at 1/3000 dilution

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 3:

C6 (Rat glioma cell line) Whole Cell Lysate at 10 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 69 kDa

Observed band size: 80 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13106

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Rat, Human, Human, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB176334

Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker

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Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (AB176334)

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Primary Antibodies

AB240191

Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free

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Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191)

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Complementary Products

Proteins & Peptides

AB268351

Recombinant Human ASH2L protein (Tagged)

SDS-PAGE - Recombinant Human ASH2L protein (Tagged) (AB268351)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASH2L also known as ASH2 is a protein with a mass of approximately 75 kDa. It functions as part of the trithorax group (TrxG) complex specifically the COMPASS-like complex where it plays an important role in histone methylation. This methylation occurs at histone H3 on lysine 4 (H3K4) an important modification for transcriptional activation. ASH2L expresses in various tissues with significant levels in the nucleus due to its involvement in chromatin modification.
Biological function summary

ASH2L participates in the regulation of gene expression. It acts as a core subunit of the SET/MLL family of H3K4 methyltransferases which form part of larger COMPASS-like complexes. These complexes modulate chromatin structure influencing transcriptional activation by methylating histone H3 at lysine 4. This modification is essential for the transition from transcriptional repression to activation during development and cell differentiation. ASH2L's role in these processes highlights its importance in maintaining proper gene expression patterns.

Pathways

ASH2L participates in the Wnt signaling pathway and influences the cell cycle. In the Wnt pathway ASH2L interacts with proteins such as β-catenin to modulate the transcription of target genes. It also affects the methylation status of promoters in various signaling pathways contributing to the regulation of gene expression involved in cell proliferation and differentiation. Additionally ASH2L is functionally connected to other proteins like MLL1 which shares involvement in chromatin remodeling and is important for epigenetic regulation.

ASH2L association with cancer and neurodevelopmental disorders is significant. Changes in ASH2L expression or function can lead to dysregulation of gene expression promoting oncogenesis or developmental abnormalities. In some cancers ASH2L's interaction with MLL can result in altered histone marks affecting tumor suppressor or oncogene expression. Besides cancer neurodevelopmental disorders like intellectual disability may have links to ASH2L due to its role in regulating genes involved in brain development. Understanding ASH2L's interactions and dysfunctions in these contexts provides insight into potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (PubMed : 12670868). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed : 19131338). Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated (PubMed : 19556245). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). May play a role in hematopoiesis (PubMed : 12670868). In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).
See full target information ASH2L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 13:869103 PubMed35911883

2022

The Quest for Anti-α-Synuclein Antibody Specificity-Lessons Learnt From Flow Cytometry Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Leupold,Veronika Sigutova,Elizaveta Gerasimova,Martin Regensburger,Sebastian Zundler,Friederike Zunke,Wei Xiang,Beate Winner,Iryna Prots
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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