AB201765

HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Aspartate Aminotransferase antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1, KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm

1 Images

Lab

Western blot - HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (AB201765)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201765 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab201765) at 1/5000 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Observed band size: 46 kDa

true

Exposure time: 4min

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
578 PE

PE Anti-Aspartate Aminotransferase +FABP-1 antibody [EPR12145]
660 APC

APC Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
Unconjugated

Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]
Carrier free

Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12145

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase also known as AST or alkaline aminotransferase is an important enzyme in amino acid metabolism. It catalyzes the transfer of an amino group from aspartate to alpha-ketoglutarate forming glutamate and oxaloacetate. This enzyme has an approximate molecular mass of 46 kDa and is expressed in the liver heart skeletal muscle and kidney tissues. AST plays a significant role in the intracellular balance of amino acids and is an important biomarker in clinical diagnostics.

Biological function summary

AST functions in the transamination process a mechanism central to the synthesis and degradation of amino acids. This process is fundamental in the production of energy and the maintenance of the urea cycle. AST does not form a part of a complex but interacts dynamically with substrates and cofactors like pyridoxal phosphate essential for its enzymatic activity. The activity of AST is important in ensuring the proper function of amino acid metabolism in various tissues.

Pathways

AST operates within the citric acid cycle and the urea cycle. In the citric acid cycle AST contributes to the interconversion of amino acids and metabolic intermediates aiding in energy production. It links to proteins such as malate dehydrogenase and glutamate dehydrogenase which play roles in associated metabolic conversions and energy cycles. These interactions reflect AST's integration in maintaining cellular metabolic processes.

AST levels serve as an indicator of liver health and are often elevated in conditions such as hepatitis and myocardial infarction. Hepatitis causes liver damage resulting in increased AST release into the bloodstream. In myocardial infarction damaged cardiac tissue releases AST providing a diagnostic cue for tissue injury. Elevated AST levels can associate with other enzymes like alanine aminotransferase (ALT) used together to assess liver and heart function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).

See full target information Aspartate aminotransferase, cytoplasmic

Additional targets

GOT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 20:2195-2209 PubMed34494941

2021

Circ-PGC increases the expression of FOXR2 by targeting miR-532-3p to promote the development of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Daokui Xia,Zhen Chen,Quan Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145]

Western blot - HRP Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (AB201765)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise