HRP Anti-beta Actin antibody [AC-15] - Loading Control

• WB

Supplier Data

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900)

All lanes:

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900) at 1/50000 dilution

Lane 1:

A549 Lysate

Lane 2:

HeLa Lysate

Lane 3:

3T3 Lysate

Lane 4:

NRK Lysate

Lane 5:

MDCK Lysate

Lane 6:

MDBK Lysate

Lane 7:

BHK Lysate

Lane 8:

COS7 Lysate

Lane 9:

CFB Lysate

Predicted band size: 41 kDa

true

• WB

AbReview17973****

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900)

Blocking Step : 5% Milk for 1 hour at 22°C.

All lanes:

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900) at 1/25000 dilution

All lanes:

Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg

Predicted band size: 41 kDa

true

This image is courtesy of an anonymous Abreview

• WB

PubMed

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900)

Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1∶1000 (ab6672), anti- IL-17 at 1∶3000, anti-TGF-β at 1∶1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.

Normalization of results was ensured by running parallel western blots with β-actin antibody at 1∶25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.

All lanes:

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900)

Predicted band size: 41 kDa

true

Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

CiteAb

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900)

Western Blotting using HRP Anti-beta Actin antibody [AC-15]HRP Anti-beta Actin antibody [AC-15], ab49900. Publication image from Harris, A. L. et al., 2014, Mol Cancer, 24517586. Legend direct from paper.

Hypoxia regulated microRNAs in MCF-7 cells. microRNA sequencing data generated from a hypoxia time course in MCF-7 (16, 32 and 48 h at 1% Oxygen) was analysed and the overlap between the microRNAs found significantly up- or down-regulated at each time point compared to normoxia control (adj.p-val <0.05) has been represented in Venn diagrams (A). The levels of HIF-1α and HIF-2α were measured by immunoblotting across the hypoxia time course (H16h, H32h, H48h) and normoxia (N), with beta-actin used as a control (B). The down-regulation of miR-4521, miR-145-3p and miR-222-5p in hypoxia was validated by qPCR (C). Fold-changes (in linear scale) obtained for each microRNA at each time point relative to normoxia control are represented in boxplots (*significant fold-change compared to normoxia after ANOVA followed by pairwise t-test, adj.p-val ≤ 0.05). The expression of miR-145-5p and miR-222-3p was also assessed by qPCR, confirming a different pattern of expression as compared to their counterpart strands.

false

• WB

CiteAb

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900)

Western Blotting using HRP Anti-beta Actin antibody [AC-15]HRP Anti-beta Actin antibody [AC-15], ab49900. Publication image from Peshkova, I. O. et al., 2019, Nat Commun, 31695038. Legend direct from paper.

Elevated expression of p53 and p21 in IL-27R-deficient HSPCs. a Scheme of experiment. b Lin− HSPCs isolated from WD-fed Apoe−/−Il27ra+/− (n = 4) and Apoe−/−Il27ra−/− (n = 4) mice infused with PBS or Apoe−/−Il27ra+/− (n = 5) and Apoe−/−Il27ra−/− (n = 4) mice infused with Ang II for 4 weeks were lysed and subjected to western blotting with p21, p53, and β-actin antibody. Each lane corresponds to individual mouse. c Quantification of WB analysis for p21 and p53, normalized to β-actin. d Scheme of experiment. e WB on Lin− HSPCs from naive Apoe−/−Il27ra+/− mice (n = 4) stimulated in vitro with Ang II, IL-27, or both for 24 h. f Quantification of WB analysis for p21. g Scheme of experiment. h Lin− HSPCs from BM of Apoe−/− (n = 4), Apoe−/−Il27ra−/− (n = 4), or Apoe−/−Il27ra−/−Trp53d/d (n = 5) mice were sorted and plated in M3434 media under myeloid conditions with or without Ang II. GM colony formation was accessed on day 6. Data are mean ± SEM from two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005, unpaired Student’s t-test (two-tailed). i Scheme describing the role of IL-27R signaling in regulation of Ang II-induced myelopoiesis and AAA. Elevation of Ang II in AAA provides a stimulus for the activation of “stress” myelopoiesis via regulation of gene expression controlling proliferation and differentiation in IL-27R-sufficient LT-HSCs. Lack of IL-27R signaling renders Ang II-primed cells unable to overcome quiescence state and significantly reduces proliferation of progenitors and myeloid cell bone marrow output, therefore reducing cell accumulation in AAA lesions and AAA progression

false

• WB

CiteAb

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900)

Western Blotting using HRP Anti-beta Actin antibody [AC-15]HRP Anti-beta Actin antibody [AC-15], ab49900. Publication image from Peshkova, I. O. et al., 2019, Nat Commun, 31695038. Legend direct from paper.

Elevated expression of p53 and p21 in IL-27R-deficient HSPCs. a Scheme of experiment. b Lin− HSPCs isolated from WD-fed Apoe−/−Il27ra+/− (n = 4) and Apoe−/−Il27ra−/− (n = 4) mice infused with PBS or Apoe−/−Il27ra+/− (n = 5) and Apoe−/−Il27ra−/− (n = 4) mice infused with Ang II for 4 weeks were lysed and subjected to western blotting with p21, p53, and β-actin antibody. Each lane corresponds to individual mouse. c Quantification of WB analysis for p21 and p53, normalized to β-actin. d Scheme of experiment. e WB on Lin− HSPCs from naive Apoe−/−Il27ra+/− mice (n = 4) stimulated in vitro with Ang II, IL-27, or both for 24 h. f Quantification of WB analysis for p21. g Scheme of experiment. h Lin− HSPCs from BM of Apoe−/− (n = 4), Apoe−/−Il27ra−/− (n = 4), or Apoe−/−Il27ra−/−Trp53d/d (n = 5) mice were sorted and plated in M3434 media under myeloid conditions with or without Ang II. GM colony formation was accessed on day 6. Data are mean ± SEM from two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005, unpaired Student’s t-test (two-tailed). i Scheme describing the role of IL-27R signaling in regulation of Ang II-induced myelopoiesis and AAA. Elevation of Ang II in AAA provides a stimulus for the activation of “stress” myelopoiesis via regulation of gene expression controlling proliferation and differentiation in IL-27R-sufficient LT-HSCs. Lack of IL-27R signaling renders Ang II-primed cells unable to overcome quiescence state and significantly reduces proliferation of progenitors and myeloid cell bone marrow output, therefore reducing cell accumulation in AAA lesions and AAA progression

false