Anti-beta Actin antibody [AC-15] is a mouse monoclonal HRP conjugated antibody that is used to detect beta Actin in Western blot. Suitable for African green monkey, Cow, Dog, Hamster, Human, Mouse, Rat samples.
- Cited in over 455 publications
- Used as a beta actin loading control antibody in western blot
- Directly labelled with HRP to save you time and simplify your experiment.
pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Tested |
African green monkey | Tested |
Cow | Tested |
Dictyostelium discoideum | Not recommended |
Dog | Tested |
Drosophila melanogaster | Not recommended |
Hamster | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/25000.00000 - 1/50000.00000 | Notes - |
Species Rat | Dilution info 1/25000.00000 - 1/50000.00000 | Notes - |
Species Hamster | Dilution info 1/25000.00000 - 1/50000.00000 | Notes - |
Species African green monkey | Dilution info 1/25000.00000 - 1/50000.00000 | Notes - |
Species Human | Dilution info 1/25000.00000 - 1/50000.00000 | Notes - |
Species Cow | Dilution info 1/25000.00000 - 1/50000.00000 | Notes - |
Species Dog | Dilution info 1/25000.00000 - 1/50000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dictyostelium discoideum, Drosophila melanogaster | Dilution info - | Notes - |
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Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Beta-actin, ACTB
Anti-beta Actin antibody [AC-15] is a mouse monoclonal HRP conjugated antibody that is used to detect beta Actin in Western blot. Suitable for African green monkey, Cow, Dog, Hamster, Human, Mouse, Rat samples.
- Cited in over 455 publications
- Used as a beta actin loading control antibody in western blot
- Directly labelled with HRP to save you time and simplify your experiment.
pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS
HRP Anti-beta Actin antibody [AC-15] (ab49900) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB in african green monkey, cow, dog, hamster, human, mouse, rat samples.
HRP Anti-beta Actin antibody [AC-15] (ab49900) has been cited over 457 times in peer reviewed journals and is trusted by the scientific community.
HRP Anti-beta Actin antibody [AC-15] (ab49900) has 10 independent reviews from customers.
HRP Anti-beta Actin antibody [AC-15] (ab49900) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in a 50 uL selling size.
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Beta Actin Western blot staining using mouse Anti-beta Actin antibody
All lanes: Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900) at 1/50000 dilution
Lane 1: A549 Lysate
Lane 2: HeLa Lysate
Lane 3: 3T3 Lysate
Lane 4: NRK Lysate
Lane 5: MDCK Lysate
Lane 6: MDBK Lysate
Lane 7: BHK Lysate
Lane 8: COS7 Lysate
Lane 9: CFB Lysate
Developed using the ECL technique.
Predicted band size: 41 kDa
Beta Actin Western blot staining using mouse Anti-beta Actin antibody
Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1∶1000 (Anti-IL-6 antibody ab6672), anti- IL-17 at 1∶3000, anti-TGF-β at 1∶1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.
Normalization of results was ensured by running parallel western blots with β-actin antibody at 1∶25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.
All lanes: Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Beta Actin Western blot staining of Whole cell lysate of Human acute monocytic leukemia THP-1. using mouse Anti-beta Actin antibody
Blocking Step: 5% Milk for 1 hour at 22°C.
All lanes: Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900) at 1/25000 dilution
All lanes: Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
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