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Anti-beta Actin antibody [AC-15] is a mouse monoclonal HRP conjugated antibody that is used to detect beta Actin in Western blot. Suitable for African green monkey, Cow, Dog, Hamster, Human, Mouse, Rat samples.

- Cited in over 455 publications
- Used as a beta actin loading control antibody in western blot
- Directly labelled with HRP to save you time and simplify your experiment.


Images

Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900), expandable thumbnail
  • Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900), expandable thumbnail
  • Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (AB49900), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
African green monkey
Tested
Cow
Tested
Dictyostelium discoideum
Not recommended
Dog
Tested
Drosophila melanogaster
Not recommended
Hamster
Tested

Tested
Tested

Species
Mouse
Dilution info
1/25000.00000 - 1/50000.00000
Notes

-

Species
Rat
Dilution info
1/25000.00000 - 1/50000.00000
Notes

-

Species
Hamster
Dilution info
1/25000.00000 - 1/50000.00000
Notes

-

Species
African green monkey
Dilution info
1/25000.00000 - 1/50000.00000
Notes

-

Species
Human
Dilution info
1/25000.00000 - 1/50000.00000
Notes

-

Species
Cow
Dilution info
1/25000.00000 - 1/50000.00000
Notes

-

Species
Dog
Dilution info
1/25000.00000 - 1/50000.00000
Notes

-

Not recommended
Not recommended

Species
Dictyostelium discoideum, Drosophila melanogaster
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

1 product for Alternative Version

Target data

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

Alternative names

Beta-actin, ACTB

Recommended products

Anti-beta Actin antibody [AC-15] is a mouse monoclonal HRP conjugated antibody that is used to detect beta Actin in Western blot. Suitable for African green monkey, Cow, Dog, Hamster, Human, Mouse, Rat samples.

- Cited in over 455 publications
- Used as a beta actin loading control antibody in western blot
- Directly labelled with HRP to save you time and simplify your experiment.

Key facts

Isotype
IgG1
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Clone number
AC-15
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

HRP Anti-beta Actin antibody [AC-15] (ab49900) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB in african green monkey, cow, dog, hamster, human, mouse, rat samples.

HRP Anti-beta Actin antibody [AC-15] (ab49900) has been cited over 457 times in peer reviewed journals and is trusted by the scientific community.

HRP Anti-beta Actin antibody [AC-15] (ab49900) has 10 independent reviews from customers.

HRP Anti-beta Actin antibody [AC-15] (ab49900) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in a 50 uL selling size.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Associated diseases and disorders

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900), expandable thumbnail

    Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900)

    Beta Actin Western blot staining using mouse Anti-beta Actin antibody

    All lanes: Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900) at 1/50000 dilution

    Lane 1: A549 Lysate

    Lane 2: HeLa Lysate

    Lane 3: 3T3 Lysate

    Lane 4: NRK Lysate

    Lane 5: MDCK Lysate

    Lane 6: MDBK Lysate

    Lane 7: BHK Lysate

    Lane 8: COS7 Lysate

    Lane 9: CFB Lysate

    Developed using the ECL technique.

    Predicted band size: 41 kDa

  • Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900), expandable thumbnail
    Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900)

    Beta Actin Western blot staining using mouse Anti-beta Actin antibody

    Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1∶1000 (Anti-IL-6 antibody ab6672), anti- IL-17 at 1∶3000, anti-TGF-β at 1∶1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.

    Normalization of results was ensured by running parallel western blots with β-actin antibody at 1∶25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.

    All lanes: Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa

  • Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900)

    Beta Actin Western blot staining of Whole cell lysate of Human acute monocytic leukemia THP-1. using mouse Anti-beta Actin antibody

    Blocking Step: 5% Milk for 1 hour at 22°C.

    All lanes: Western blot - HRP Anti-beta Actin antibody [AC-15] - Loading Control (ab49900) at 1/25000 dilution

    All lanes: Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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