Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ACTB.
Images
![Western blot - HRP Anti-beta Actin antibody [BA3R] (AB173838), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-ba3r-ab173838--western-blot-img13125.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-beta Actin antibody [BA3R] (AB173838)")
![Western blot - HRP Anti-beta Actin antibody [BA3R] (AB173838), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-ba3r-ab173838--western-blot-img165131.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-beta Actin antibody [BA3R] (AB173838)")
Publications
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- The Journal of clinical investigation 132:2022Degradation of GSPT1 causes TP53-independent cell death in leukemia while sparing normal hematopoietic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRob S Sellar,Adam S Sperling,Mikołaj Słabicki,Jessica A Gasser,Marie E McConkey,Katherine A Donovan,Nada Mageed,Dylan N Adams,Charles Zou,Peter G Miller,Ravi K Dutta,Steffen Boettcher,Amy E Lin,Brittany Sandoval,Vanessa A Quevedo Barrios,Veronica Kovalcik,Jonas Koeppel,Elizabeth K Henderson,Emma C Fink,Lu Yang,Anthony Chan,Sheela Pangeni Pokharel,Erik J Bergstrom,Rajan Burt,Namrata D Udeshi,Steven A Carr,Eric S Fischer,Chun-Wei Chen,Benjamin L EbertPubMed 35763353
- Journal of cellular and molecular medicine 24:12736-127492020Small RNA sequencing reveals a novel tsRNA-06018 playing an important role during adipogenic differentiation of hMSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Wang et. al.PubMed 32939933
- Stem cell research & therapy 11:3652020A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Wang et. al.PubMed 32831139
- Molecular medicine reports 19:4719-47262019Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Wang et. al.PubMed 31059010
- Oncology reports 41:246-2562018Bromodomain PHD‑finger transcription factor promotes glioma progression and indicates poor prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanling Pan,Feng Yuan,Yijie Li,Guoping Wang,Zhiren Lin,Longhua ChenPubMed 30542695
- Molecular and cellular biochemistry 449:295-3032018miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingnuan Li et. al.PubMed 29959592
- Oncology reports 40:909-9152018Alternative splicing variant of RHBDD2 is associated with cell stress response and breast cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRomina Canzoneri et. al.PubMed 29901166
- Cell stem cell 21:195-208.e62017Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartha S Windrem et. al.PubMed 28736215
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human ACTB. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| African green monkey | Tested |
| Dog | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species African green monkey | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Dog | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ACTB.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human ACTB. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
- Clone number
- BA3R
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
- Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
- Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
- Associated diseases and disorders
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-ba3r-ab173838--western-blot-img13125.jpg "Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)")
Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)
4-20% Tris-HCl polyacrylamide gel.
All lanes: Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838) at 1/1000 dilution
Lane 1: HeLa cell lysate at 30 µg
Lane 2: 293T cell lysate at 30 µg
Lane 3: U2OS cell lysate at 30 µg
Lane 4: COS7 cell lysate at 30 µg
Lane 5: NIH 3T3 cell lysate at 30 µg
Lane 6: NRK cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
![Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-ba3r-ab173838--western-blot-img165131.jpg "Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)")
Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838)
Blocking buffer: 5% milk.All lanes: Western blot - HRP Anti-beta Actin antibody [BA3R] (ab173838) at 1 µg
Lane 1: A549 (Human alveolar basal epithelial cells) at 30 µg
Lane 2: COS-7 (African green monkey kidney fibroblast cells) at 30 µg
Lane 3: MDCK (Madin-Darby Canine Kidney cells) at 30 µg
Lane 4: C2C12 (Mouse myoblast cells) at 30 µg
Lane 5: MDA-MB-231 (Human breast cancer cells) at 30 µg
Lane 6: RSC96 at 30 µg
Lane 7: Mouse lung tissue lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 42 kDa
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