AB173838

HRP Anti-beta Actin antibody [BA3R] - Loading Control

Name: HRP Anti-beta Actin antibody [BA3R] - Loading Control (ab173838) | Abcam
Brand: Abcam Limited
SKU: AB173838
Price: 535 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ACTB.

View Alternative Names

Beta-actin, ACTB

2 Images

Supplier Data

Western blot - HRP Anti-beta Actin antibody [BA3R] - Loading Control (AB173838)

Blocking buffer : 5% milk.

All lanes:

Western blot - HRP Anti-beta Actin antibody [BA3R] - Loading Control (ab173838) at 1 µg

Lane 1:

A549 (Human alveolar basal epithelial cells) at 30 µg

Lane 2:

COS-7 (African green monkey kidney fibroblast cells) at 30 µg

Lane 3:

MDCK (Madin-Darby Canine Kidney cells) at 30 µg

Lane 4:

C2C12 (Mouse myoblast cells) at 30 µg

Lane 5:

MDA-MB-231 (Human breast cancer cells) at 30 µg

Lane 6:

RSC96 at 30 µg

Lane 7:

Mouse lung tissue lysate at 30 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

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Supplier Data

Western blot - HRP Anti-beta Actin antibody [BA3R] - Loading Control (AB173838)

4-20% Tris-HCl polyacrylamide gel.

All lanes:

Western blot - HRP Anti-beta Actin antibody [BA3R] - Loading Control (ab173838) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

293T cell lysate at 30 µg

Lane 3:

U2OS cell lysate at 30 µg

Lane 4:

COS7 cell lysate at 30 µg

Lane 5:

NIH 3T3 cell lysate at 30 µg

Lane 6:

NRK cell lysate at 30 µg

Predicted band size: 41 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BA3R

Isotype

IgG2b

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

applications

Immunogen

Synthetic Peptide within Human ACTB. The exact immunogen used to generate this antibody is proprietary information.

P60709

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 132: PubMed35763353

2022

Degradation of GSPT1 causes TP53-independent cell death in leukemia while sparing normal hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rob S Sellar,Adam S Sperling,Mikołaj Słabicki,Jessica A Gasser,Marie E McConkey,Katherine A Donovan,Nada Mageed,Dylan N Adams,Charles Zou,Peter G Miller,Ravi K Dutta,Steffen Boettcher,Amy E Lin,Brittany Sandoval,Vanessa A Quevedo Barrios,Veronica Kovalcik,Jonas Koeppel,Elizabeth K Henderson,Emma C Fink,Lu Yang,Anthony Chan,Sheela Pangeni Pokharel,Erik J Bergstrom,Rajan Burt,Namrata D Udeshi,Steven A Carr,Eric S Fischer,Chun-Wei Chen,Benjamin L Ebert

Journal of cellular and molecular medicine 24:12736-12749 PubMed32939933

2020

Small RNA sequencing reveals a novel tsRNA-06018 playing an important role during adipogenic differentiation of hMSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Lingling Cao,Shan He,Kai Long,Xinping Wang,Hui Yu,Baicheng Ma,Xiaoyuan Xu,Weidong Li

Stem cell research & therapy 11:365 PubMed32831139

2020

A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Jun Mei,Xingnuan Li,Xiaoyuan Xu,Baicheng Ma,Weidong Li

Molecular medicine reports 19:4719-4726 PubMed31059010

2019

Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Ruiqiao Yan,Xiaoyuan Xu,Huan Yu,Jianfang Wu,Yaofang Yang,Weidong Li

Oncology reports 41:246-256 PubMed30542695

2018

Bromodomain PHD‑finger transcription factor promotes glioma progression and indicates poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling Pan,Feng Yuan,Yijie Li,Guoping Wang,Zhiren Lin,Longhua Chen

Molecular and cellular biochemistry 449:295-303 PubMed29959592

2018

miR-377-3p regulates adipogenic differentiation of human bone marrow mesenchymal stem cells by regulating LIFR.

Applications

Unspecified application

Species

Unspecified reactive species

Xingnuan Li,Yaofang Yang,Ruiqiao Yan,Xiaoyuan Xu,Liyun Gao,Jun Mei,Jianyun Liu,Xinping Wang,Jie Zhang,Ping Wu,Weidong Li,Zhijun Zhao,Jianjun Xiong,Tao Wang

Oncology reports 40:909-915 PubMed29901166

2018

Alternative splicing variant of RHBDD2 is associated with cell stress response and breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Canzoneri,Martín Enrique Rabassa,Agustina Gurruchaga,Valeria Ferretti,Sabina Palma,Marina Isla-Larrain,María Virginia Croce,Ezequiel Lacunza,Martín Carlos Abba

Cell stem cell 21:195-208.e6 PubMed28736215

2017

Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Martha S Windrem,Mikhail Osipovitch,Zhengshan Liu,Janna Bates,Devin Chandler-Militello,Lisa Zou,Jared Munir,Steven Schanz,Katherine McCoy,Robert H Miller,Su Wang,Maiken Nedergaard,Robert L Findling,Paul J Tesar,Steven A Goldman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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HRP Anti-beta Actin antibody [BA3R] - Loading Control

Western blot - HRP Anti-beta Actin antibody [BA3R] - Loading Control (AB173838)

Western blot - HRP Anti-beta Actin antibody [BA3R] - Loading Control (AB173838)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reagents, Controls & Accessories

Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise