Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.
Images
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (AB197277), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8224-loading-control-ab197277--western-blot-img56950.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (AB197277)")
Publications
Filter by
- Bioscience reports 42:2022Histomorphological and ultrastructural cadmium-induced kidney injuries and precancerous lesions in rats and screening for biomarkers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXichen Wan,Zelong Xing,Jin Ouyang,Hui Liu,Chengquan Cheng,Ting Luo,Shiqun Yu,Li Meihua,Shaoxin HuangPubMed 35678542
- Frontiers in neurology 12:7509082021Preconditioning With Intermittent Hypobaric Hypoxia Attenuates Stroke Damage and Modulates Endocytosis in Residual Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaqi Wan,Lu Huang,Yanmin Liu,Weizhong Ji,Changxing Li,Ri-Li GePubMed 34975719
- EMBO molecular medicine 13:e144592021Brain cross-protection against SARS-CoV-2 variants by a lentiviral vaccine in new transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Wen Ku,Pierre Authié,Maryline Bourgine,François Anna,Amandine Noirat,Fanny Moncoq,Benjamin Vesin,Fabien Nevo,Jodie Lopez,Philippe Souque,Catherine Blanc,Ingrid Fert,Sébastien Chardenoux,Llta Lafosse,Delphine Cussigh,David Hardy,Kirill Nemirov,Françoise Guinet,Francina Langa Vives,Laleh Majlessi,Pierre CharneauPubMed 34647691
- Cell host & microbe 29:236-249.e62020Intranasal vaccination with a lentiviral vector protects against SARS-CoV-2 in preclinical animal models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Wen Ku,Maryline Bourgine,Pierre Authié,Jodie Lopez,Kirill Nemirov,Fanny Moncoq,Amandine Noirat,Benjamin Vesin,Fabien Nevo,Catherine Blanc,Philippe Souque,Houda Tabbal,Emeline Simon,David Hardy,Marine Le Dudal,Françoise Guinet,Laurence Fiette,Hugo Mouquet,François Anna,Annette Martin,Nicolas Escriou,Laleh Majlessi,Pierre CharneauPubMed 33357418
- EBioMedicine 56:1027922020Comparative exome sequencing reveals novel candidate genes for retinitis pigmentosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Yi et. al.PubMed 32454406
- Frontiers in immunology 10:26182019Late-Onset Antibody Deficiency Due to Monoallelic Alterations in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Schröder et. al.PubMed 31803180
- Haematologica 105:1925-19362019Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichela Colombo et. al.PubMed 31582544
- Cell death & disease 10:5962019Folate can promote the methionine-dependent reprogramming of glioblastoma cells towards pluripotency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRacha Zgheib et. al.PubMed 31395852
- Biochemistry 57:1262-12732018A Small-Molecule Inhibitor of Human DNA Polymerase η Potentiates the Effects of Cisplatin in Tumor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaroof K Zafar et. al.PubMed 29345908
- Pathology oncology research : POR 24:427-4382017Development of Novel Monoclonal Antibodies for Evaluation of Transmembrane Prostate Androgen-Induced Protein 1 (TMEPAI) Expression Patterns in Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMikhail S Karbyshev,Evgeniya S Grigoryeva,Viktor V Volkomorov,Elisabeth Kremmer,Alexander Huber,Irina V Mitrofanova,Evgeniya V Kaigorodova,Marina V Zavyalova,Julia G Kzhyshkowska,Nadezda V Cherdyntseva,Evgeny L ChoynzonovPubMed 28584893
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Chinese hamster | Predicted |
| Cow | Predicted |
| Drosophila melanogaster | Predicted |
| Schizosaccharomyces pombe | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5% BSA. Incubate antibody in TBST for one hour or more. |
Species Rat | Dilution info 1 µg/mL | Notes This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5% BSA. Incubate antibody in TBST for one hour or more. |
Species Human | Dilution info 1 µg/mL | Notes This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5% BSA. Incubate antibody in TBST for one hour or more. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Drosophila melanogaster, Schizosaccharomyces pombe, Chinese hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.
Key facts
- Isotype
- IgG3
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- mAbcam 8224
- Purification technique
- Affinity purification
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
- Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
- Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
- Associated diseases and disorders
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8224-loading-control-ab197277--western-blot-img56950.jpg "Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277)")
Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197277 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277) at 1/5000 dilution
Lane 1: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 42 kDa
Exposure time: 10s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com