HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control
5
(1 Review)
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(12 Publications)
Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.
View Alternative Names
Beta-actin, ACTB
- WB
Lab
Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (AB197277)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197277 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
All lanes:
Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277) at 1/5000 dilution
Lane 1:
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 2:
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3:
PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4:
Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg
Predicted band size: 41 kDa
Observed band size: 42 kDa
true
Exposure time: 10s
Related conjugates and formulations (2)
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Anti-beta Actin antibody [mAbcam 8224] - Loading Control
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Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free
Reactivity data
Properties and storage information
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Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
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Target data
Publications (12)
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Nature cell biology 26:1571-1584 PubMed39117797
2024
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Bioscience reports 42: PubMed35678542
2022
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Frontiers in neurology 12:750908 PubMed34975719
2021
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EMBO molecular medicine 13:e14459 PubMed34647691
2021
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Cell host & microbe 29:236-249.e6 PubMed33357418
2020
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EBioMedicine 56:102792 PubMed32454406
2020
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Frontiers in immunology 10:2618 PubMed31803180
2019
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Haematologica 105:1925-1936 PubMed31582544
2019
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Cell death & disease 10:596 PubMed31395852
2019
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Biochemistry 57:1262-1273 PubMed29345908
2018
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Product promise
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