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AB197277

HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control

5

(1 Review)

|

(12 Publications)

Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

View Alternative Names

Beta-actin, ACTB

1 Images
Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (AB197277)
  • WB

Lab

Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (AB197277)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197277 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277) at 1/5000 dilution

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

Exposure time: 10s

  • Unconjugated

    Anti-beta Actin antibody [mAbcam 8224] - Loading Control

  • Carrier free

    Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 8224

Isotype

IgG3

Light chain type

kappa

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
See full target information ACTB

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 26:1571-1584 PubMed39117797

2024

Spermidine is essential for fasting-mediated autophagy and longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian J Hofer,Ioanna Daskalaki,Martina Bergmann,Jasna Friščić,Andreas Zimmermann,Melanie I Mueller,Mahmoud Abdellatif,Raffaele Nicastro,Sarah Masser,Sylvère Durand,Alexander Nartey,Mara Waltenstorfer,Sarah Enzenhofer,Isabella Faimann,Verena Gschiel,Thomas Bajaj,Christine Niemeyer,Ilias Gkikas,Lukas Pein,Giulia Cerrato,Hui Pan,YongTian Liang,Jelena Tadic,Andrea Jerkovic,Fanny Aprahamian,Christine E Robbins,Nitharsshini Nirmalathasan,Hansjörg Habisch,Elisabeth Annerer,Frederik Dethloff,Michael Stumpe,Franziska Grundler,Françoise Wilhelmi de Toledo,Daniel E Heinz,Daniela A Koppold,Anika Rajput Khokhar,Andreas Michalsen,Norbert J Tripolt,Harald Sourij,Thomas R Pieber,Rafael de Cabo,Mark A McCormick,Christoph Magnes,Oliver Kepp,Joern Dengjel,Stephan J Sigrist,Nils C Gassen,Simon Sedej,Tobias Madl,Claudio De Virgilio,Ulrich Stelzl,Markus H Hoffmann,Tobias Eisenberg,Nektarios Tavernarakis,Guido Kroemer,Frank Madeo

Bioscience reports 42: PubMed35678542

2022

Histomorphological and ultrastructural cadmium-induced kidney injuries and precancerous lesions in rats and screening for biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Xichen Wan,Zelong Xing,Jin Ouyang,Hui Liu,Chengquan Cheng,Ting Luo,Shiqun Yu,Li Meihua,Shaoxin Huang

Frontiers in neurology 12:750908 PubMed34975719

2021

Preconditioning With Intermittent Hypobaric Hypoxia Attenuates Stroke Damage and Modulates Endocytosis in Residual Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Wan,Lu Huang,Yanmin Liu,Weizhong Ji,Changxing Li,Ri-Li Ge

EMBO molecular medicine 13:e14459 PubMed34647691

2021

Brain cross-protection against SARS-CoV-2 variants by a lentiviral vaccine in new transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Wen Ku,Pierre Authié,Maryline Bourgine,François Anna,Amandine Noirat,Fanny Moncoq,Benjamin Vesin,Fabien Nevo,Jodie Lopez,Philippe Souque,Catherine Blanc,Ingrid Fert,Sébastien Chardenoux,Llta Lafosse,Delphine Cussigh,David Hardy,Kirill Nemirov,Françoise Guinet,Francina Langa Vives,Laleh Majlessi,Pierre Charneau

Cell host & microbe 29:236-249.e6 PubMed33357418

2020

Intranasal vaccination with a lentiviral vector protects against SARS-CoV-2 in preclinical animal models.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Wen Ku,Maryline Bourgine,Pierre Authié,Jodie Lopez,Kirill Nemirov,Fanny Moncoq,Amandine Noirat,Benjamin Vesin,Fabien Nevo,Catherine Blanc,Philippe Souque,Houda Tabbal,Emeline Simon,David Hardy,Marine Le Dudal,Françoise Guinet,Laurence Fiette,Hugo Mouquet,François Anna,Annette Martin,Nicolas Escriou,Laleh Majlessi,Pierre Charneau

EBioMedicine 56:102792 PubMed32454406

2020

Comparative exome sequencing reveals novel candidate genes for retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yi,Jiamin Ouyang,Wenmin Sun,Shiqiang Li,Xueshan Xiao,Qingjiong Zhang

Frontiers in immunology 10:2618 PubMed31803180

2019

Late-Onset Antibody Deficiency Due to Monoallelic Alterations in .

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Schröder,Georgios Sogkas,Manfred Fliegauf,Thilo Dörk,Di Liu,Leif G Hanitsch,Sophie Steiner,Carmen Scheibenbogen,Roland Jacobs,Bodo Grimbacher,Reinhold E Schmidt,Faranaz Atschekzei

Haematologica 105:1925-1936 PubMed31582544

2019

Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Colombo,Silvia Garavelli,Mara Mazzola,Natalia Platonova,Domenica Giannandrea,Raffaella Colella,Luana Apicella,Marialuigia Lancellotti,Elena Lesma,Silvia Ancona,Maria Teresa Palano,Marzia Barbieri,Elisa Taiana,Elisa Lazzari,Andrea Basile,Mauro Turrini,Anna Pistocchi,Antonino Neri,Raffaella Chiaramonte

Cell death & disease 10:596 PubMed31395852

2019

Folate can promote the methionine-dependent reprogramming of glioblastoma cells towards pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Racha Zgheib,Shyue-Fang Battaglia-Hsu,Sébastien Hergalant,Maelle Quéré,Jean-Marc Alberto,Céline Chéry,Pierre Rouyer,Guillaume Gauchotte,Jean-Louis Guéant,Farès Namour

Biochemistry 57:1262-1273 PubMed29345908

2018

A Small-Molecule Inhibitor of Human DNA Polymerase η Potentiates the Effects of Cisplatin in Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maroof K Zafar,Leena Maddukuri,Amit Ketkar,Narsimha R Penthala,Megan R Reed,Sarah Eddy,Peter A Crooks,Robert L Eoff
View all publications

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