Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 296 publications.
Images
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8226-loading-control-ab20272--western-blot-img85835.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)")
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8226-loading-control-ab20272--western-blot-img97403.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)")
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8226-loading-control-ab20272--western-blot-img123201.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)")
Publications
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- Endocrine regulations 57:279-2912023Nicotinamide prevention in diabetes-induced alterations in the rat liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamara Kuchmerovska,Lesya Yanitska,Oksana Horkunenko,Mykhailo Guzyk,Tetiana Tykhonenko,Irina PryvrotskaPubMed 38127690
- Nanomaterials (Basel, Switzerland) 13:2023Intravenous Injection of PEI-Decorated Iron Oxide Nanoparticles Impacts NF-kappaB Protein Expression in Immunologically Stressed Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Schwarz,Julia Göring,Cordula Grüttner,Ingrid HilgerPubMed 38133063
- Viruses 15:2023Identification of Host Factors for Rift Valley Fever Phlebovirus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVelmurugan Balaraman,Sabarish V Indran,Yonghai Li,David A Meekins,Laxmi U M R Jakkula,Heidi Liu,Micheal P Hays,Jayme A Souza-Neto,Natasha N Gaudreault,Philip R Hardwidge,William C Wilson,Friedemann Weber,Juergen A RichtPubMed 38005928
- Experimental & molecular medicine 55:2332-23452023Lactobacillus plantarum ameliorates NASH-related inflammation by upregulating L-arginine production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Yun Kim,Jun Yong Park,Heon Yung GeePubMed 37907736
- iScience 26:1081432023Oral vancomycin treatment suppresses gut trypsin activity and preserves intestinal barrier function during EAE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Bianchimano,Kacper Iwanowski,Emma M Smith,Adam Cantor,Paola Leone,Gerold Bongers,Carlos G Gonzalez,Yoon Hongsup,Joshua Elias,Howard L Weiner,Jose C Clemente,Stephanie K TankouPubMed 37915599
- Journal of biochemical and molecular toxicology 37:e235102023NOD2 silencing promotes cell apoptosis and inhibits drug resistance in chronic lymphocytic leukemia by inhibiting the NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCuiping Zheng,Zongsi Zhu,Shanshan Weng,Qikai Zhang,Yixiao Fu,Xiaoping Cai,Zhen Liu,Yuejian ShiPubMed 37700718
- Antioxidants (Basel, Switzerland) 12:2023Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNikoletta Almási,Szilvia Török,Amin Al-Awar,Médea Veszelka,László Király,Denise Börzsei,Renáta Szabó,Csaba VargaPubMed 37627526
- Nutrients 15:2023OLL2712 Induces Autophagy via MYD88 and Strengthens Tight Junction Integrity to Promote the Barrier Function in Intestinal Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumiko Watanabe-Yasuoka,Ayako Gotou,Shigeomi Shimizu,Toshihiro SashiharaPubMed 37375559
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 131:313-3242023Circ_0006873 suppresses the osteogenic differentiation of human-derived mesenchymal stem cells through mediating miR-20a/SMURF2 axis in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiangna Pang,Yongfu Wu,Yanlin Ji,Yilan Si,Fang LiangPubMed 37185991
- Nutrients 15:2023Sesamin Metabolites Suppress the Induction of Cellular Senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChie Araki,Daisuke Takemoto,Yoshinori Kitagawa,Norifumi Tateishi,Tomohiro Rogi,Takayuki Izumo,Shimpei Kawamoto,Hiroshi Shibata,Eiji Hara,Masaaki NakaiPubMed 37049468
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| African green monkey | Predicted |
| Chicken | Predicted |
| Chinese hamster | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Drosophila melanogaster | Predicted |
| Guinea pig | Predicted |
| Horse | Predicted |
| Monkey | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Sheep | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details. |
Species Rat | Dilution info 1/5000 | Notes Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details. |
Species Human | Dilution info 1/5000 | Notes Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Drosophila melanogaster, Monkey, African green monkey, Chinese hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
2 products for Alternative Version
Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Mouse Monoclonal beta Actin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 296 publications.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
mAbcam 8226
- Purity
IgG fraction
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
- Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
- Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
- Associated diseases and disorders
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8226-loading-control-ab20272--western-blot-img85835.jpg "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)")
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
All lanes: Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1: human testis lysate at 20 µg
Lane 2: human brain lysate at 20 µg
Lane 3: human tonsil lysate at 20 µg
Lane 4: mouse brain lysate at 20 µg
Lane 5: mouse spleen lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Exposure time: 2min
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8226-loading-control-ab20272--western-blot-img97403.jpg "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)")
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
All lanes: Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: 3T3 whole cell lysate at 20 µg
Lane 3: Rat brain lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 42 kDa
Exposure time: 30s
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272), expandable thumbnail](https://content.abcam.com/products/images/hrp-beta-actin-antibody-mabcam-8226-loading-control-ab20272--western-blot-img123201.jpg "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)")
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
All lanes: Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lanes 1 and 5: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 2 and 6: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lanes 3 and 7: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lanes 4 and 8: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 42 kDa
Exposure time: 1min
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