AB235761

HRP Anti-beta Tubulin antibody [AA2] - Loading Control

Name: Anti-beta Tubulin HRP antibody [AA2] (ab235761) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB235761
Price: 545 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Beta-5-tubulin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Tubulin beta-5 chain, TUBB5

1 Images

Lab

Western blot - HRP Anti-beta Tubulin antibody [AA2] - Loading Control (AB235761)

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 50 mins before being transferred onto a nitrocellulose membrane at 30V for 70 mins. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab235761 overnight at 4°C. Antibody binding was visualized using ECL development solution ab133406.

Samples : 1. HeLa (20μg) 2. NIH 3T3 (20μg) 3. PC12 (20μg) 4. HepG2 (20μg).

All lanes:

Western blot - HRP Anti-beta Tubulin antibody [AA2] - Loading Control (ab235761)

Predicted band size: 49 kDa

false

Unconjugated

Anti-beta Tubulin antibody [AA2] - Loading Control
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-beta Tubulin antibody [AA2] - Microtubule Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AA2

Isotype

IgG1

Light chain type

kappa

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Recognizes amino acids 416-431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed : 2207090, PubMed : 6504138, PubMed : 7704569). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 2207090, PubMed : 6504138, PubMed : 7704569).

See full target information TUBB5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadj3656 PubMed38457497

2024

Large-FOV 3D localization microscopy by spatially variant point spread function generation.

Applications

Unspecified application

Species

Unspecified reactive species

Dafei Xiao,Reut Kedem Orange,Nadav Opatovski,Amit Parizat,Elias Nehme,Onit Alalouf,Yoav Shechtman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-beta Tubulin antibody [AA2] - Loading Control

Western blot - HRP Anti-beta Tubulin antibody [AA2] - Loading Control (AB235761)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise