AB185057

HRP Anti-beta Tubulin antibody [EPR1330] - Loading Control

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal Beta-5-tubulin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

View Alternative Names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

1 Images

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Western blot - HRP Anti-beta Tubulin antibody [EPR1330] - Loading Control (AB185057)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185057 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406

All lanes:

Western blot - HRP Anti-beta Tubulin antibody [EPR1330] - Loading Control (ab185057) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 49 kDa

Observed band size: 52 kDa

true

Exposure time: 2s

578 PE

PE Anti-beta Tubulin antibody [EPR1330] – Microtubule Marker
660 APC

APC Anti-beta Tubulin antibody [EPR1330] – Microtubule Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR1330] – Microtubule Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR1330] – Microtubule Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-beta Tubulin antibody [EPR1330] – Microtubule Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-beta Tubulin antibody [EPR1330] – Microtubule Marker
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-beta Tubulin antibody [EPR1330] - Microtubule Marker
Unconjugated

Anti-beta Tubulin antibody [EPR1330] - Loading Control
Carrier free

Anti-beta Tubulin antibody [EPR1330] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1330

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29020 PubMed40781473

2025

Suicide gene therapy targeting ewing sarcoma via an ewing-specific GGAA promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Saint T Cervera,Selene Martínez,María Iranzo-Martínez,Raquel M Melero-Fernández de Mera,Javier Alonso

Cancer gene therapy 32:437-449 PubMed40089636

2025

Targeted inactivation of EWSR1 : : FLI1 gene in Ewing sarcoma via CRISPR/Cas9 driven by an Ewing-specific GGAA promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Saint T Cervera,Selene Martínez,María Iranzo-Martínez,Laura Notario,Raquel M Melero-Fernández de Mera,Javier Alonso

Cancers 13: PubMed34359682

2021

Therapeutic Potential of Inactivation by CRISPR/Cas9 in Ewing Sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Saint T Cervera,Carlos Rodríguez-Martín,Enrique Fernández-Tabanera,Raquel M Melero-Fernández de Mera,Matias Morin,Sergio Fernández-Peñalver,Maria Iranzo-Martínez,Jorge Amhih-Cardenas,Laura García-García,Laura González-González,Miguel Angel Moreno-Pelayo,Javier Alonso

ASN neuro 11:1759091419833502 PubMed30862176

2019

Astrocyte and Neuronal Pannexin1 Contribute Distinctly to Seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Eliana Scemes,Libor Velíšek,Jana Velíšková

Oncoimmunology 5:e1221557 PubMed27853646

2016

Hepatocellular carcinoma is accelerated by NASH involving M2 macrophage polarization mediated by hif-1induced IL-10.

Applications

Unspecified application

Species

Unspecified reactive species

Aditya Ambade,Abhishek Satishchandran,Banishree Saha,Benedek Gyongyosi,Patrick Lowe,Karen Kodys,Donna Catalano,Gyongyi Szabo

Scientific reports 6:21340 PubMed26888602

2016

Alcoholic hepatitis accelerates early hepatobiliary cancer by increasing stemness and miR-122-mediated HIF-1α activation.

Applications

Species

Unspecified reactive species

Aditya Ambade,Abhishek Satishchandran,Gyongyi Szabo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-beta Tubulin antibody [EPR1330] - Loading Control

Western blot - HRP Anti-beta Tubulin antibody [EPR1330] - Loading Control (AB185057)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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