AB21058

HRP Anti-beta Tubulin antibody - Loading Control

Name: Anti-beta Tubulin - Loading Control HRP antibody (ab21058) IgG | Abcam
Brand: Abcam Limited
SKU: AB21058
Price: 435 USD
Availability: InStock
Rating: 5 (4 reviews)

BOND RX™ Validated
What is this?

(4 Reviews)

(155 Publications)

Rabbit Polyclonal Beta-5-tubulin antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 155 publications.

View Alternative Names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

3 Images

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta Tubulin antibody - Loading Control (AB21058)

IHC image of beta Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue performed on a Leica BOND^TM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21058, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Project

Western blot - HRP Anti-beta Tubulin antibody - Loading Control (AB21058)

All lanes:

Western blot - HRP Anti-beta Tubulin antibody - Loading Control (ab21058) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - A-431 whole cell lysate (<a href='/en-us/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Predicted band size: 49 kDa

Observed band size: 50 kDa

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Project1099****

Western blot - HRP Anti-beta Tubulin antibody - Loading Control (AB21058)

All lanes:

Western blot - HRP Anti-beta Tubulin antibody - Loading Control (ab21058) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 5:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 6:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Predicted band size: 49 kDa

Observed band size: 51 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB

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Publications (155)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1563969 PubMed40936697

2025

ERK3/MAPK6 promotes triple-negative breast cancer progression through collective migration and EMT plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Morazzo,Soraia Fernandes,Marina Fortea,Helena Skálová,Daniel Pereira-Sousa,Marco Cassani,Kamila Vrzalová,Filip Kafka,Jan Vrbský,Mathilde Soulez,Sylvain Meloche,Ole Morten-Seternes,Veronika Bosáková,Jaeyoung Shin,Jan Frič,Kristina Haase,Giancarlo Forte

International journal of molecular sciences 26: PubMed40806549

2025

Assessing Determinants of Response to PARP Inhibition in Germline Mutant Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonora Allavena,Michela Croce,Bruna Dalmasso,Cecilia Profumo,Valentina Rigo,Virginia Andreotti,Irene Vanni,Benedetta Pellegrino,Antonino Musolino,Nicoletta Campanini,William Bruno,Luca Mastracci,Gabriele Zoppoli,Enrica Teresa Tanda,Francesco Spagnolo,Paola Ghiorzo,Lorenza Pastorino

Frontiers in endocrinology 16:1615103 PubMed40747314

2025

A key role of the PGC-1α/ERR-α pathway in regulation of angiogenic factors in proliferative diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Mohd I Nawaz,Ajmal Ahmad,Mairaj M Siddiquei,Eef Allegaert,Priscilla W Gikandi,Gert De Hertogh,Ghislain Opdenakker

Journal of thoracic disease 17:4249-4263 PubMed40688308

2025

regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an model of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Yimeng Zhou,Lianzhi Zhang,Wenjia Zhang,Qunlin Gong,Zhong Zhang,Nan Xu,Ashley R Wilson-Smith,Nannan Chen

International journal of molecular sciences 26: PubMed40564963

2025

Co-Aggregation of Syndecan-3 with β-Amyloid Aggravates Neuroinflammation and Cognitive Impairment in 5×FAD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Ye,Mingfeng Li,Min Liu,Xinghan Wu,Fan Tian,Yanju Gong,Yan Cao,Jingtai Zhang,Xueling Zhang,Chuan Qin,Ling Zhang

Cells 14: PubMed39851513

2025

ADAMTS13 Improves Endothelial Function and Reduces Inflammation in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Mohd I Nawaz,Ajmal Ahmad,Mairaj Siddiquei,Eef Allegaert,Lowie Adyns,Lotte Vanbrabant,Priscilla W Gikandi,Gert De Hertogh,Sofie Struyf,Ghislain Opdenakker

Metabolomics : Official journal of the Metabolomic Society 21:16 PubMed39832080

2025

Wall shear stress modulates metabolic pathways in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Simões-Faria,Margo Daems,Hanna M Peacock,Mathias Declercq,Anton Willems,Elizabeth A V Jones,Bart Ghesquière

International journal of molecular sciences 25: PubMed39684779

2024

A 3D-Printable Cell Array for In Vitro Breast Cancer Modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Arciero,Silvia Buonvino,Valeria Palumbo,Manuel Scimeca,Sonia Melino

PLoS biology 22:e3002895 PubMed39527653

2024

Mitochondrial calcium uptake orchestrates vertebrate pigmentation via transcriptional regulation of keratin filaments.

Applications

Unspecified application

Species

Unspecified reactive species

Jyoti Tanwar,Kriti Ahuja,Akshay Sharma,Paras Sehgal,Gyan Ranjan,Farina Sultan,Anushka Agrawal,Donato D'Angelo,Anshu Priya,Vamsi K Yenamandra,Archana Singh,Anna Raffaello,Muniswamy Madesh,Rosario Rizzuto,Sridhar Sivasubbu,Rajender K Motiani

Journal of neuroinflammation 21:282 PubMed39487531

2024

Interleukin-6 deficiency reduces neuroinflammation by inhibiting the STAT3-cGAS-STING pathway in Alzheimer's disease mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min Liu,Jirong Pan,Xiaomeng Li,Xueling Zhang,Fan Tian,Mingfeng Li,Xinghan Wu,Ling Zhang,Chuan Qin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-beta Tubulin antibody - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta Tubulin antibody - Loading Control (AB21058)

Western blot - HRP Anti-beta Tubulin antibody - Loading Control (AB21058)

Western blot - HRP Anti-beta Tubulin antibody - Loading Control (AB21058)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (155)

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