Rabbit Polyclonal Beta-5-tubulin antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 126 publications.
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- The Journal of clinical investigation 134:2023AEP-cleaved DDX3X induces alternative RNA splicing events to mediate cancer cell adaptation in harsh microenvironments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenrui Zhang,Lu Cao,Jian Yang,Shuai Zhang,Jianyi Zhao,Zhonggang Shi,Keman Liao,Haiwei Wang,Binghong Chen,Zhongrun Qian,Haoping Xu,Linshi Wu,Hua Liu,Hongxiang Wang,Chunhui Ma,Yongming Qiu,Jianwei Ge,Jiayi Chen,Yingying LinPubMed 37988165
- Cell death & disease 14:7552023NINJ1 is activated by cell swelling to regulate plasma membrane permeabilization during regulated necrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYves Dondelinger,Dario Priem,Jon Huyghe,Tom Delanghe,Peter Vandenabeele,Mathieu J M BertrandPubMed 37980412
- Diabetology & metabolic syndrome 15:2172023Glucose fluctuations aggravate myocardial fibrosis via activating the CaMKII/Stat3 signaling in type 2 diabtetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhang,Huan-Huan Liu,Fan Yang,Zhi-Yuan Zhang,Zhen-Ye Zhang,Xiao-Xi Zhao,Ling-Ling Qian,Shi-Peng Dang,Ru-Xing WangPubMed 37891701
- Frontiers in molecular biosciences 10:12503352023Primary cilia-mediated regulation of microglial secretion in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeungeun Yeo,Jaemyung Jang,Hyun Jin Jung,Hyeyoung Lee,Youngshik ChoePubMed 37942288
- Cancers 15:2023A Combinatorial Regulatory Platform Determines Expression of RNA Polymerase III Subunit RPC7α () in Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiying Cheng,Sihang Zhou,Rajendra K C,Simon Lizarazo,Leela Mouli,Anshita Jayanth,Qing Liu,Kevin Van BortlePubMed 37894362
- Diabetes, metabolic syndrome and obesity : targets and therapy 16:3045-30562023Advanced Glycation End Products Downregulate Connexin 43 and Connexin 40 in Diabetic Atrial Myocytes via the AMPK Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Yang,Huan-Huan Liu,Lei Zhang,Xiao-Lu Zhang,Jie Zhang,Feng Li,Ning Zhao,Zhi-Yuan Zhang,Qi Kong,Xiao-Yu Liu,Ying Wu,Zhi-Ming Yu,Ling-Ling Qian,Ru-Xing WangPubMed 37810573
- Genes 14:2023Aicardi Syndrome Is a Genetically Heterogeneous Disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThuong T Ha,Rosemary Burgess,Morgan Newman,Ching Moey,Simone A Mandelstam,Alison E Gardner,Atma M Ivancevic,Duyen Pham,Raman Kumar,Nicholas Smith,Chirag Patel,Stephen Malone,Monique M Ryan,Sophie Calvert,Clare L van Eyk,Michael Lardelli,Samuel F Berkovic,Richard J Leventer,Linda J Richards,Ingrid E Scheffer,Jozef Gecz,Mark A CorbettPubMed 37628618
- JCI insight 8:2023Endothelial-derived FABP4 constitutes the majority of basal circulating hormone and regulates lipolysis-driven insulin secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren E Inouye,Kacey J Prentice,Alexandra Lee,Zeqiu B Wang,Carla Dominguez-Gonzalez,Mu Xian Chen,Jillian K Riveros,M Furkan Burak,Grace Y Lee,Gökhan S HotamışlıgilPubMed 37279064
- Journal of Cancer 14:1837-18472023Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhimmada Hatthakarnkul,Aula Ammar,Kathryn A F Pennel,Leah Officer-Jones,Silvia Cusumano,Jean A Quinn,Amna Ahmed Mohemmed Matly,Peter G Alexander,Jennifer Hay,Ditte Andersen,Gerard Lynch,Hester C van Wyk,Noori Maka,Donald C McMillan,John Le Quesne,Chanitra Thuwajit,Joanne EdwardsPubMed 37476187
- Nature communications 14:34032023Variants in SART3 cause a spliceosomopathy characterised by failure of testis development and neuronal defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatie L Ayers,Stefanie Eggers,Ben N Rollo,Katherine R Smith,Nadia M Davidson,Nicole A Siddall,Liang Zhao,Josephine Bowles,Karin Weiss,Ginevra Zanni,Lydie Burglen,Shay Ben-Shachar,Jenny Rosensaft,Annick Raas-Rothschild,Anne Jørgensen,Ralf B Schittenhelm,Cheng Huang,Gorjana Robevska,Jocelyn van den Bergen,Franca Casagranda,Justyna Cyza,Svenja Pachernegg,David K Wright,Melanie Bahlo,Alicia Oshlack,Terrence J O'Brien,Patrick Kwan,Peter Koopman,Gary R Hime,Nadine Girard,Chen Hoffmann,Yuval Shilon,Amnon Zung,Enrico Bertini,Mathieu Milh,Bochra Ben Rhouma,Neila Belguith,Anu Bashamboo,Kenneth McElreavey,Ehud Banne,Naomi Weintrob,Bruria BenZeev,Andrew H SinclairPubMed 37296101
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Xenopus laevis, Chimpanzee, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Xenopus laevis, Chimpanzee, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain
Recommended products
Rabbit Polyclonal Beta-5-tubulin antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 126 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.
- Biological function summary
This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.
- Pathways
Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.
- Associated diseases and disorders
Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - HRP Anti-beta Tubulin antibody - Loading Control (ab21058)
All lanes: Western blot - HRP Anti-beta Tubulin antibody - Loading Control (ab21058) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
Lane 4: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 5: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 7: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta Tubulin antibody - Loading Control (ab21058)
IHC image of beta Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21058, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - HRP Anti-beta Tubulin antibody - Loading Control (ab21058)
All lanes: Western blot - HRP Anti-beta Tubulin antibody - Loading Control (ab21058) at 1/1000 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa
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