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AB196707

HRP Anti-BNIP3 antibody [ANa40]

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(1 Publication)

Mouse Monoclonal BNIP3 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NIP3, BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

1 Images
Western blot - HRP Anti-BNIP3 antibody [ANa40] (AB196707)
  • WB

Lab

Western blot - HRP Anti-BNIP3 antibody [ANa40] (AB196707)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196707 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-BNIP3 antibody [ANa40] (ab196707) at 1/5000 dilution

All lanes:

Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

true

Exposure time: 2s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ANa40

Isotype

IgG2b

Light chain type

kappa

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP : in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
See full target information BNIP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7930 PubMed40855028

2025

Dual mitotic bookmarking by GAF and H3K27ac orchestrates differential propagation of cell fate memory in neural development.

Applications

Unspecified application

Species

Unspecified reactive species

Rulan Zhang,Jie Liu,Zimo Zhang,Zili Chen,Tanpeng Wang,Yuying Shen,Zejun Lan,Jingyi Chu,Haoxuan Tang,Xiyue Zhang,Yan Song
View all publications
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