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AB7637

HRP Anti-Bovine Serum Albumin antibody

4

(3 Reviews)

|

(1 Publication)

Anti-Bovine Serum Albumin antibody - HRP conjugated (ab7637) is a rabbit polyclonal antibody detecting Bovine Serum Albumin in Western Blot, ELISA, Dot Blot and Immunomicroscopy. Suitable for Cow.

- Trusted since 2001

View Alternative Names

Albumin, BSA, ALB

1 Images
Western blot - HRP Anti-Bovine Serum Albumin antibody (AB7637)
  • WB

Supplier Data

Western blot - HRP Anti-Bovine Serum Albumin antibody (AB7637)

Blocking Buffer : 5% BLOTTO in TBS.

All lanes:

Western blot - HRP Anti-Bovine Serum Albumin antibody (ab7637) at 1/1000 dilution

Lane 1:

Bovine Serum Albumin reduced at 0.5 µg

Lane 3:

Bovine Serum Albumin non-reduced at 0.5 µg

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Cow

Applications

Dot, WB, ELISA, IM

applications

Immunogen

Native Full Length Protein corresponding to Cow Albumin.

P02769

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Alternative Products

Primary Antibodies

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Anti-Bovine Serum Albumin antibody [5F9]

Western blot - Anti-Bovine Serum Albumin antibody [5F9] (AB9092)

  • 0
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Primary Antibodies

AB79827

Anti-Bovine Serum Albumin antibody [1C12]

Western blot - Anti-Bovine Serum Albumin antibody [1C12] (AB79827)

  • 0
  • 0
Primary Antibodies

AB240330

Anti-Albumin antibody [EPR12774] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [EPR12774] - BSA and Azide free (AB240330)

  • 0
  • 0
Primary Antibodies

AB192603

Anti-Albumin antibody [EPR12774]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Albumin antibody [EPR12774] (AB192603)

  • 0
  • 0

Complementary Products

Assay Kits

AB235628

Albumin (BCG) Assay Kit (Colorimetric)

Functional Studies - Albumin (BCG) Assay Kit (Colorimetric) (AB235628)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB191338

HRP Anti-Streptavidin antibody

  • 0
  • 0
Assay Kits

AB179887

Human Albumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Albumin ELISA Kit (AB179887)

  • 3
  • 1
Primary Antibodies

AB37382

Chicken IgY, polyclonal - Isotype Control (BSA and Azide free)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IM" : {"fullname" : "Immunomicroscopy", "shortname":"IM"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Cow": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/5000 - 1/20000", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IM-species-checked": "guaranteed", "IM-species-dilution-info": "", "IM-species-notes": "<p></p>" } } }
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Product details

Product Specifications
HRP Anti-Bovine Serum Albumin antibody (ab7637) is validated for use in Dot blot, ELISA, IM, WB in cow samples.
HRP Anti-Bovine Serum Albumin antibody (ab7637) specifically detects Bovine Serum Albumin (UniProt ID: P02768) and is sold in 50 ug selling sizes.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Anti-BOVINE ALBUMIN (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
Preservative: 0.01% Gentamicin sulphate Constituents: 1% Polyethylene glycol, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.
Biological function summary

Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.

Pathways

Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.

Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed : 22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information Albumin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 7:1528109 PubMed30357008

2018

Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales.

Applications

WB

Species

Unspecified reactive species

Anna-Kristin Ludwig,Kyra De Miroschedji,Thorsten R Doeppner,Verena Börger,Johannes Ruesing,Vera Rebmann,Stephan Durst,Sören Jansen,Michel Bremer,Elmar Behrmann,Bernhard B Singer,Holger Jastrow,Jan Dominik Kuhlmann,Fouzi El Magraoui,Helmut E Meyer,Dirk M Hermann,Bertram Opalka,Stefan Raunser,Matthias Epple,Peter A Horn,Bernd Giebel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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