HRP Anti-BRD2 antibody [EPR7642]

• WB

Lab

Western blot - HRP Anti-BRD2 antibody [EPR7642] (AB198536)

ab198536 was shown to specifically react with BRD2 in wild-type HAP1 cells as signal was lost in BRD2 knockout cells. Wild-type and BRD2 knockout samples were subjected to SDS-PAGE. ab198536 was incubated overnight at 4°C at 1/5000 dilution. Blots were developed with ECL technique.

All lanes:

Western blot - HRP Anti-BRD2 antibody [EPR7642] (ab198536) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

BRD2 knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 88 kDa

Observed band size: 110 kDa

true

Exposure time: 8min

• WB

Lab

Western blot - HRP Anti-BRD2 antibody [EPR7642] (AB198536)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198536 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-BRD2 antibody [EPR7642] (ab198536) at 1/5000 dilution

Lane 1:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

NCCIT (Human pluripotent embryonal teratocarcinoma) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Predicted band size: 88 kDa

Observed band size: 110 kDa

true

Exposure time: 8min