JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB220507

HRP Anti-BrdU antibody [BU1/75 (ICR1)]

Be the first to review this product! Submit a review

|

(1 Publication)

Rat Monoclonal BrdU antibody - conjugated to HRP. Suitable for IHC-P and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

BUdr, Bromodeoxyuridine

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (AB220507)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (AB220507)

IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded BrdU treated rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (AB220507)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-BrdU antibody [BU1/75 (ICR1)] (AB220507)

Negative IHC image of BrdU staining in a section of formalin-fixed paraffin-embedded normal rat skin, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab220507, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

BU1/75 (ICR1)

Isotype

IgG2a

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

IHC-P

applications

Specificity

This antibody reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not react with thymidine. It has been reported in the literature that this antibody clone cross-reacts with Edu (PMID: 23272138) and some customers reported that it cross reacts with IdU.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB125306

BrdU Immunohistochemistry Kit

3

2 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available here.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information BrdU
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 9:756755 PubMed34746108

2021

Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ibrahim Fathi,Takehiro Imura,Akiko Inagaki,Yasuhiro Nakamura,Ayman Nabawi,Masafumi Goto
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB152095

Anti-BrdU antibody

primary-antibodies

brdu-antibody-ab152095

4

(4 reviews)

Alternative Product
Primary Antibodies

AB8955

Anti-BrdU antibody [IIB5]

primary-antibodies

brdu-antibody-iib5-ab8955

4

(3 reviews)

Alternative Product
Primary Antibodies

AB136650

Anti-BrdU antibody [Bu20a]

primary-antibodies

brdu-antibody-bu20a-ab136650

0

(0 reviews)

Alternative Product
Primary Antibodies

AB8152

Anti-BrdU antibody [IIB5]

primary-antibodies

brdu-antibody-iib5-ab8152

5

(7 reviews)

Alternative Product
Primary Antibodies

AB2284

Biotin Anti-BrdU antibody

primary-antibodies

biotin-brdu-antibody-ab2284

5

(6 reviews)

Alternative Product
Primary Antibodies

AB8039

Anti-BrdU antibody [MoBu-1]

primary-antibodies

brdu-antibody-mobu-1-ab8039

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com