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AB205818

HRP Anti-c-Myc antibody [Y69]

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(7 Publications)

Rabbit Recombinant Monoclonal C-Myc/MYC antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

1 Images
Western blot - HRP Anti-c-Myc antibody [Y69] (AB205818)
  • WB

Lab

Western blot - HRP Anti-c-Myc antibody [Y69] (AB205818)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205818 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-c-Myc antibody [Y69] (ab205818) at 1/5000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Predicted band size: 48 kDa

Observed band size: 58 kDa

true

Exposure time: 4min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y69

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

If you need other conjugated anti-c-myc (Y69) RabMAb antibodies, find our range of products here.

We also offer a PBS only version of this clone as product ab168727.

For more information on choosing the right c-Myc antibody for you, please visit Antibodies to c-Myc and Myc tag.

To support you in your research, we offer a wider range of tools to study c-Myc and Myc-tagged proteins.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).
See full target information MYC
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Genes 15: PubMed39457457

2024

Combinatorial Anti-Cancer Effect of Polypurine Reverse Hoogsteen Hairpins against and Targeting in Prostate and Pancreatic Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Simonas Valiuska,Kayla K Elder,Steven J McKay,Carlos J Ciudad,Véronique Noé,Tracy A Brooks

American journal of cancer research 13:2188-2200 PubMed37293180

2023

Eukaryotic initiation factor 3b regulates the development and progression of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhui Li,Jianhua Zhao,Zhikun Yuan,Dehan Yuan,Mao Fang,Minru Xie,Minghan Xia

International journal of molecular sciences 24: PubMed36613820

2022

Targeting MYC Regulation with Polypurine Reverse Hoogsteen Oligonucleotides.

Applications

Unspecified application

Species

Unspecified reactive species

Simonas Valiuska,Alexandra Maria Psaras,Véronique Noé,Tracy A Brooks,Carlos J Ciudad

Molecular cancer therapeutics 21:1524-1534 PubMed35877475

2022

Decreased DNA Damage and Improved p53 Specificity of RITA Analogs.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhan,Xiaolei Zhou,Sylvain Peuget,Madhurendra Singh,Brian D Peyser,Zhimin Fan,Galina Selivanova

RNA (New York, N.Y.) 27:1577-1588 PubMed34535544

2021

DDX3X and DDX3Y are redundant in protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Srivats Venkataramanan,Margaret Gadek,Lorenzo Calviello,Kevin Wilkins,Stephen N Floor

Developmental cell 56:1989-2006.e6 PubMed34118203

2021

Oncogene-regulated release of extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Seda Kilinc,Rebekka Paisner,Roman Camarda,Suprit Gupta,Olga Momcilovic,Rebecca A Kohnz,Baris Avsaroglu,Noelle D L'Etoile,Rushika M Perera,Daniel K Nomura,Andrei Goga

Scientific reports 9:8006 PubMed31142756

2019

HIV Infection Induces Extracellular Cathepsin B Uptake and Damage to Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yisel M Cantres-Rosario,Sarah C Ortiz-Rodríguez,Aemil G Santos-Figueroa,Marines Plaud,Karla Negron,Bianca Cotto,Dianne Langford,Loyda M Melendez
View all publications
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Product promise

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