Rabbit Recombinant Monoclonal CALM3 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse samples.
Images
![Western blot - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (AB199558), expandable thumbnail](https://content.abcam.com/products/images/hrp-calmodulin-1-2-3-antibody-ep799y-ab199558--western-blot-img95145.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (AB199558)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (AB199558), expandable thumbnail](https://content.abcam.com/products/images/hrp-calmodulin-1-2-3-antibody-ep799y-ab199558--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28015.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (AB199558)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Calmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding (PubMed:16760425, PubMed:31454269). Calcium-binding is required for the activation of calmodulin (PubMed:16760425, PubMed:31454269, PubMed:35568036). Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases, such as myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), and phosphatases (PubMed:16760425, PubMed:35568036). Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425).(Microbial infection) Required for C.violaceum CopC and S.flexneri OspC3 arginine ADP-riboxanase activity.
Alternative names
CALML2, CAM3, CAMC, CAMIII, CALM3, CAMIII, CAMC, CAM3, CALML2, Calmodulin-3
Recommended products
Rabbit Recombinant Monoclonal CALM3 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP799Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calmodulin also known as calmoduline is a multifunctional intermediate calcium-binding protein expressed widely in eukaryotic cells. This small protein has a molecular weight of approximately 16.7 kDa. Calmodulin 1 2 and 3 serve as the key isoforms facilitating a variety of calcium-mediated processes. You can find it in cellular contexts where calcium signaling plays a regulatory role. Due to its ubiquity it acts as a mediator in numerous cellular responses.
- Biological function summary
Calmodulin modulates intracellular activities by binding to calcium ions and interacting with a wide array of target proteins. It is not part of a molecular complex but can partner with proteins like kinases and phosphatases leading to downstream effects on diverse cellular processes. Calmodulin function extends to muscle contraction cell division and signal transduction impacting both basal and specialized cellular functions.
- Pathways
Calmodulin operates as a functional link in calcium signaling and the MAPK signaling pathways. In these pathways calmodulin influences the activity of proteins such as myosin light-chain kinase and calmodulin-dependent protein kinase. These interactions showcase calmodulin's pivotal role in translating calcium signaling into cellular responses connecting several signaling cascades.
- Associated diseases and disorders
Calmodulin has associations with certain cardiovascular diseases and neurodegenerative conditions. Its relationship with ion channel dysfunction links it to disorders like long QT syndrome. Additionally calmodulin interacts with proteins like sodium-calcium exchangers or other ion-handling channels reflecting its involvement in maintaining cellular ionic balance and consequently its implication in pathological states when dysregulated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (ab199558), expandable thumbnail](https://content.abcam.com/products/images/hrp-calmodulin-1-2-3-antibody-ep799y-ab199558--western-blot-img95145.jpg "Western blot - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (ab199558)")
Western blot - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (ab199558)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199558 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (ab199558) at 1/1000 dilution
Lane 1: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 18 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (ab199558), expandable thumbnail](https://content.abcam.com/products/images/hrp-calmodulin-1-2-3-antibody-ep799y-ab199558--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28015.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (ab199558)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Calmodulin 1/2/3 antibody [EP799Y] (ab199558)
IHC image of calmodulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab199558, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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