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AB305816

HRP Anti-Cardiac Troponin I antibody [EP1106Y]

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Rabbit Recombinant Monoclonal Cardiac Troponin I antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EP1106Y] (AB305816)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EP1106Y] (AB305816)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Cardiac Troponin I with ab305816 at 1/100 (5.0 µg/mL) dilution. Negative control : no staining on human skeletal muscle. The section was incubated with ab305816 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EP1106Y] (AB305816)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cardiac Troponin I antibody [EP1106Y] (AB305816)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Cardiac Troponin I with ab305816 at 1/100 (5.0 µg/mL) dilution. Cytoplasmic staining on human cardiac muscle. The section was incubated with ab305816 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • 578 PE

    PE Anti-Cardiac Troponin I antibody [EP1106Y]

  • 660 APC

    APC Anti-Cardiac Troponin I antibody [EP1106Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Cardiac Troponin I antibody [EP1106Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Cardiac Troponin I antibody [EP1106Y]

  • Unconjugated

    Anti-Cardiac Troponin I antibody [EP1106Y]

  • Carrier free

    Anti-Cardiac Troponin I antibody [EP1106Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1106Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB200016

Human Cardiac Troponin I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)

  • 5
  • 2
Assay Kits

AB200016

Human Cardiac Troponin I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)

  • 5
  • 2
Proteins & Peptides

AB283299

Recombinant Human Cardiac Troponin I protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cardiac Troponin I protein (His tag N-Terminus) (AB283299)

  • 0
  • 0
Primary Antibodies

AB209813

Anti-Cardiac Troponin T antibody [EPR20266]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [EPR20266] (AB209813)

  • 5
  • 2
Proteins & Peptides

AB316587

Recombinant Human Cardiac Troponin T Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Cardiac Troponin T Protein Standard (His tag) (AB316587)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNI3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com