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AB323778

HRP Anti-Cathepsin K antibody [EP9403]

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Rabbit Recombinant Monoclonal Cathepsin K antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

CTSO, CTSO2, CTSK, Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X

2 Images
Western blot - HRP Anti-Cathepsin K antibody [EP9403] (AB323778)
  • WB

Supplier Data

Western blot - HRP Anti-Cathepsin K antibody [EP9403] (AB323778)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - HRP Anti-Cathepsin K antibody [EP9403] (ab323778) at 1/1000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Observed band size: 39 kDa,36 kDa

false

Exposure time: 15s

Western blot - HRP Anti-Cathepsin K antibody [EP9403] (AB323778)
  • WB

Supplier Data

Western blot - HRP Anti-Cathepsin K antibody [EP9403] (AB323778)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - HRP Anti-Cathepsin K antibody [EP9403] (ab323778) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Observed band size: 39 kDa,36 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP9403

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB65303

Cathepsin K Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin K Activity Assay Kit (Fluorometric) (AB65303)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin K also known as CTSK is a protease enzyme that belongs to the papain family of cysteine proteases. With a molecular weight of approximately 37 kDa Cathepsin K is mainly expressed in osteoclasts which are cells involved in bone resorption. This enzyme functions by cleaving collagen a vital component of bone extracellular matrix which facilitates bone remodeling and turnover. Known for its collagenolytic activity Cathepsin K is important in processes where breakdown of collagen fibers is required.
Biological function summary

Cathepsin K plays a significant role in bone metabolism. It operates within the osteoclasts where it degrades type I collagen and other matrix proteins essential for normal bone maintenance and repair. Cathepsin K does not function as part of a complex but acts independently in its enzymatic role. It is highly specific to substrates found in the bone matrix differentiating it from other cathepsins like cathepsin L or B that may have broader expression and role.

Pathways

Cathepsin K is an important player in the bone remodeling pathway. This pathway involves a series of coordinated actions between osteoclasts and osteoblasts to maintain bone health. Cathepsin K's activity is tightly regulated within this pathway to ensure proper bone density and structure. It is associated with other proteins such as osteopontin and matrix metalloproteinases which work together to modulate bone turnover. Within the RANK/RANKL pathway Cathepsin K acts as a downstream effector following osteoclast activation by RANKL.

Cathepsin K is most notably linked to osteoporosis and pycnodysostosis. Osteoporosis arises due to excessive bone resorption where elevated Cathepsin K activity leads to weakened bone structure increasing fracture risk. Pycnodysostosis is a rare genetic disorder caused by mutations in the gene coding for Cathepsin K resulting in impaired bone resorption and abnormally dense but brittle bones. In these conditions proteins such as RANKL and osteoprotegerin also play critical roles by influencing osteoclast differentiation and activity thereby modulating Cathepsin K's effects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed : 11082042).
See full target information CTSK
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com