AB195017

HRP Anti-CD34 antibody [EP373Y]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CD34 antibody - conjugated to HRP. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD34, Hematopoietic progenitor cell antigen CD34

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD34 antibody [EP373Y] (AB195017)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD34 antibody [EP373Y] (AB195017)

IHC image of CD34 staining in a section of formalin-fixed paraffin-embedded normal human umbilical cord tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195017 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

578 PE

PE Anti-CD34 antibody [EP373Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD34 antibody [EP373Y]
660 APC

APC Anti-CD34 antibody [EP373Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD34 antibody [EP373Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD34 antibody [EP373Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD34 antibody [EP373Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD34 antibody [EP373Y]
Unconjugated

Anti-CD34 antibody [EP373Y]
Carrier free

Anti-CD34 antibody [EP373Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP373Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/hrp-rabbit-igg-monoclonal-epr25a-isotype-control-ab199507'>ab199507</a> - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

See full target information CD34

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36983022

2023

dCas9-Based PDGFR-β Activation ADSCs Accelerate Wound Healing in Diabetic Mice through Angiogenesis and ECM Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Yumeng Li,Deyong Li,Lu You,Tian Deng,Qiuyu Pang,Xiangmin Meng,Bingmei Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-CD34 antibody [EP373Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD34 antibody [EP373Y] (AB195017)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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