AB194981

HRP Anti-CD41 antibody [EPR4330]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CD41 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

CD41, GP2B, ITGAB, ITGA2B, Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb

1 Images

Lab

Western blot - HRP Anti-CD41 antibody [EPR4330] (AB194981)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194981 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CD41 antibody [EPR4330] (ab194981) at 1/5000 dilution

All lanes:

Platelet (Human) Whole Cell Lysate at 10 µg

Predicted band size: 113 kDa

Observed band size: 113 kDa

true

Exposure time: 20min

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD41 antibody [EPR4330]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD41 antibody [EPR4330]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD41 antibody [EPR4330]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD41 antibody [EPR4330]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD41 antibody [EPR4330]
Unconjugated

Anti-CD41 antibody [EPR4330]
Carrier free

Anti-CD41 antibody [EPR4330] - BSA and Azide free
Carrier free

Anti-CD41 antibody [EPR4330] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4330

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-IIb/beta-3 (ITGA2B : ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface (By similarity). Integrin ITGA2B : ITGB3 is also the receptor of erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717).

See full target information ITGA2B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 72:517-544 PubMed39215640

2024

Making Multiplexed Imaging Flexible: Combining Essential Markers With Established Antibody Panels.

Applications

Unspecified application

Species

Unspecified reactive species

Ashik Jawahar Deen,Johan Thorsson,Eleanor M O'Roberts,Pranauti Panshikar,Tony Ullman,David Krantz,Carolina Oses,Charlotte Stadler

Pharmaceuticals (Basel, Switzerland) 14: PubMed34451836

2021

GMP-Compliant Radiosynthesis of [F]GP1, a Novel PET Tracer for the Detection of Thrombi.

Applications

Unspecified application

Species

Unspecified reactive species

Verena Hugenberg,Marion Zerna,Mathias Berndt,Reinhard Zabel,Rainer Preuss,Dirk Rolfsmeier,Janet Wegener,Henrik Fox,Astrid Kassner,Hendrik Milting,Norman Koglin,Andrew W Stephens,Jan F Gummert,Wolfgang Burchert,Marcus-André Deutsch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-CD41 antibody [EPR4330]

Western blot - HRP Anti-CD41 antibody [EPR4330] (AB194981)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise