HRP Anti-CD44 antibody [EPR1013Y]

• WB

Lab

Western blot - HRP Anti-CD44 antibody [EPR1013Y] (AB194989)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab194989 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989) at 1/2500 dilution

All lanes:

TF-1 Whole Cell Lysate at 10 µg

Predicted band size: 81 kDa

Observed band size: 82 kDa

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Exposure time: 20min

• WB

Lab

Western blot - HRP Anti-CD44 antibody [EPR1013Y] (AB194989)

Lanes 1 - 4 : Merged signal (red and green). Green - ab194989 observed at 80 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab194989 was shown to react with CD44 (HRP) in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab194989 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 2500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - HRP Anti-CD44 antibody [EPR1013Y] (ab194989) at 1/2500 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

CD44 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CD44 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cd44-knockout-hela-cell-line-ab262515'>ab262515</a>)

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

Predicted band size: 81 kDa

Observed band size: 80 kDa

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