Rabbit Recombinant Monoclonal CDC37 phospho S13 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.
Images
![Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (AB202364), expandable thumbnail](https://content.abcam.com/products/images/hrp-cdc37-phospho-s13-antibody-epr4879-ab202364--western-blot-img43065.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (AB202364)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206).
Alternative names
CDC37A, CDC37, Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit, p50Cdc37
Recommended products
Rabbit Recombinant Monoclonal CDC37 phospho S13 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4879
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Cdc37 protein also known as cell division cycle 37 homolog is a molecular chaperone that assists in the proper folding and stabilization of protein kinases. With a mass of approximately 44 kDa Cdc37 is widely expressed in mammalian tissues. It interacts directly with other proteins aiding in their stabilization and activity. Functioning closely with Hsp90 Cdc37 forms a chaperone complex critical for the maturation of several protein kinases.
- Biological function summary
Cdc37 serves as a co-chaperone with Hsp90 in regulating protein kinase activity by maintaining their functional conformation and stability. This protein acts mainly as a kinase-specific adapter that recruits kinases to the Hsp90 chaperone system forming a protein complex essential for the proper functioning of key signaling molecules. Its role is vital in signal transduction and cellular growth regulation ensuring proper cell cycle progression and response to external stimuli.
- Pathways
Cdc37 plays a significant role in the PI3K/AKT and MAPK signaling pathways. In these pathways Cdc37 assists in the proper activation and function of various kinases such as AKT and RAF which are important for cell growth survival and proliferation. Its partnership with Hsp90 in these pathways underlines its importance in maintaining kinase activity ensuring that signaling cascades proceed correctly for normal cellular function.
- Associated diseases and disorders
Cdc37 has been implicated in cancer and neurodegenerative diseases like Alzheimer's. Overexpression or dysfunction of Cdc37 can lead to improper activation of oncogenic kinases contributing to tumor progression. Additionally the protein's association with Hsp90 highlights its potential involvement in Alzheimer's where protein misfolding and stability are disrupted making Cdc37 a target of interest for therapeutic intervention in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364), expandable thumbnail](https://content.abcam.com/products/images/hrp-cdc37-phospho-s13-antibody-epr4879-ab202364--western-blot-img43065.jpg "Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)")
Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)
Cdc37 (phospho S13) Western blot staining using rabbit Anti-Cdc37 (phospho S13) antibody
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-p53 (phospho S46) antibody [EP42Y] ab202864 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364) at 1/5000 dilution
Lane 1: Untreated HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) whole cell lysate serum starved and Calyculin A treated at 10 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) whole cell lysate, serum starved and Calyculin A treated, then membrane was then incubated with Alkaline phosphatase at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 3min
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