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Rabbit Recombinant Monoclonal CDC42 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - HRP Anti-CDC42 antibody [EPR15620] (AB215810), expandable thumbnail
  • Western blot - HRP Anti-CDC42 antibody [EPR15620] (AB215810), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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5 products for Alternative Version

Target data

Function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed:15642749). Regulates cell migration (PubMed:17038317, PubMed:22843693). In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed:14978216). Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed:33523862). Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed:26465210). Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation (PubMed:33310911).

Alternative names

Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42

Recommended products

Rabbit Recombinant Monoclonal CDC42 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15620
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - HRP Anti-CDC42 antibody [EPR15620] (ab215810), expandable thumbnail

    Western blot - HRP Anti-CDC42 antibody [EPR15620] (ab215810)

    ab215810 was shown to recognize CDC42 in wild-type HAP1 cells as signal was lost at the expected MW in CDC42 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab215810 and Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    All lanes: Western blot - HRP Anti-CDC42 antibody [EPR15620] (ab215810) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: CDC42 knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 21 kDa

    Exposure time: 20min

  • Western blot - HRP Anti-CDC42 antibody [EPR15620] (ab215810), expandable thumbnail

    Western blot - HRP Anti-CDC42 antibody [EPR15620] (ab215810)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215810 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-CDC42 antibody [EPR15620] (ab215810) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 4: Spleen (Mouse) Tissue Lysate at 10 µg

    Lane 5: Spleen (Rat) Tissue Lysate at 10 µg

    Lane 6: HAP1 WT at 20 µg

    Lane 7: CDC42 knockout HAP1 at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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