Rat Monoclonal p16INK4A antibody - conjugated to HRP. Suitable for WB and reacts with Mouse samples. Cited in 4 publications.
Images
![Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (AB202225), expandable thumbnail](https://content.abcam.com/products/images/hrp-cdkn2a-p19arf-antibody-5-c3-ab202225--western-blot-img69592.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (AB202225)")
Publications
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- Clinical science (London, England : 1979) 135:1873-18952021Brahma-related gene-1 promotes tubular senescence and renal fibrosis through Wnt/β-catenin/autophagy axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWangqiu Gong,Congwei Luo,Fenfen Peng,Jing Xiao,Yiqun Zeng,Bohui Yin,Xiaowen Chen,Shuting Li,Xiaoyang He,Yanxia Liu,Huihui Cao,Jiangping Xu,Haibo LongPubMed 34318888
- Physiological reports 9:e146962021Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Miao,Jiewu Huang,Congwei Luo,Huiyun Ye,Xian Ling,Qinyu Wu,Weiwei Shen,Lili ZhouPubMed 33463897
- Aging cell 18:e130042019Wnt/β-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Miao et. al.PubMed 31318148
- Journal of the American Society of Nephrology : JA 29:1238-12562018Wnt9a Promotes Renal Fibrosis by Accelerating Cellular Senescence in Tubular Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCongwei Luo et. al.PubMed 29440280
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Not recommended |
| Mouse | Tested |
| Hamster | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Hamster, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Alternative names
P16ink4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4
Recommended products
Rat Monoclonal p16INK4A antibody - conjugated to HRP. Suitable for WB and reacts with Mouse samples. Cited in 4 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 5-C3
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225), expandable thumbnail](https://content.abcam.com/products/images/hrp-cdkn2a-p19arf-antibody-5-c3-ab202225--western-blot-img69592.jpg "Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)")
Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202225 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225) at 1/5000 dilution
All lanes: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 19 kDa
Exposure time: 8min
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