AB202225

HRP Anti-CDKN2A/p19ARF antibody [5-C3]

Name: Anti-CDKN2A/p19ARF HRP antibody [5-C3] (ab202225) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB202225
Price: 565 USD
Availability: InStock

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(6 Publications)

Rat Monoclonal p16INK4A antibody - conjugated to HRP. Suitable for WB and reacts with Mouse samples. Cited in 6 publications.

View Alternative Names

P16ink4a, Cdkn2a, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, p16-INK4a, CDK4I, p16-INK4

1 Images

Lab

Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (AB202225)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202225 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225) at 1/5000 dilution

All lanes:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Predicted band size: 18 kDa

Observed band size: 19 kDa

true

Exposure time: 8min

Unconjugated

Anti-CDKN2A/p19ARF antibody [5-C3]
Carrier free

Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

5-C3

Isotype

IgG2b

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

See full target information Cdkn2a

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18075 PubMed38213100

2024

C-X-C chemokine receptor type 4 promotes tubular cell senescence and renal fibrosis through β-catenin-inhibited fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Qinyu Wu,Qiurong Chen,Dan Xu,Xiaoxu Wang,Huiyun Ye,Xiaolong Li,Yabing Xiong,Jiemei Li,Shan Zhou,Jinhua Miao,Weiwei Shen,Youhua Liu,Hongxin Niu,Ying Tang,Lili Zhou

Journal of cellular and molecular medicine 25:8957-8972 PubMed34414658

2021

Cannabinoid receptor 2 plays a central role in renal tubular mitochondrial dysfunction and kidney ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Zhou,Xian Ling,Ping Meng,Ye Liang,Kunyu Shen,Qinyu Wu,Yunfang Zhang,Qiyan Chen,Shuangqin Chen,Youhua Liu,Lili Zhou

Clinical science (London, England : 1979) 135:1873-1895 PubMed34318888

2021

Brahma-related gene-1 promotes tubular senescence and renal fibrosis through Wnt/β-catenin/autophagy axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wangqiu Gong,Congwei Luo,Fenfen Peng,Jing Xiao,Yiqun Zeng,Bohui Yin,Xiaowen Chen,Shuting Li,Xiaoyang He,Yanxia Liu,Huihui Cao,Jiangping Xu,Haibo Long

Physiological reports 9:e14696 PubMed33463897

2021

Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Miao,Jiewu Huang,Congwei Luo,Huiyun Ye,Xian Ling,Qinyu Wu,Weiwei Shen,Lili Zhou

Aging cell 18:e13004 PubMed31318148

2019

Wnt/β-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Miao,Jiafeng Liu,Jing Niu,Yunfang Zhang,Weiwei Shen,Congwei Luo,Yahong Liu,Chuanjiang Li,Hongyan Li,Peiliang Yang,Youhua Liu,Fan Fan Hou,Lili Zhou

Journal of the American Society of Nephrology : JA 29:1238-1256 PubMed29440280

2018

Wnt9a Promotes Renal Fibrosis by Accelerating Cellular Senescence in Tubular Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Congwei Luo,Shan Zhou,Zhanmei Zhou,Yahong Liu,Li Yang,Jiafeng Liu,Yunfang Zhang,Hongyan Li,Youhua Liu,Fan Fan Hou,Lili Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-CDKN2A/p19ARF antibody [5-C3]

Western blot - HRP Anti-CDKN2A/p19ARF antibody [5-C3] (AB202225)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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