JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB203736

HRP Anti-CLCA1 antibody [EPR12254-88]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal CLCA1 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CACC1, CLCA1, Calcium-activated chloride channel regulator 1, Calcium-activated chloride channel family member 1, Calcium-activated chloride channel protein 1, hCLCA1, CaCC-1, hCaCC-1

1 Images
Western blot - HRP Anti-CLCA1 antibody [EPR12254-88] (AB203736)
  • WB

Lab

Western blot - HRP Anti-CLCA1 antibody [EPR12254-88] (AB203736)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203736 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CLCA1 antibody [EPR12254-88] (ab203736) at 1/1000 dilution

All lanes:

Small Intestine (Human) Tissue Lysate at 10 µg

Predicted band size: 100 kDa

Observed band size: 85 kDa

true

Exposure time: 16min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12254-88

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB174319

Anti-CLCA1 antibody [EPR12254]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (AB174319)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB205828

Alexa Fluor® 647 Anti-EGFR (phospho Y1068) antibody [EP774Y]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB205828)

  • 0
  • 0
Primary Antibodies

AB202755

Alexa Fluor® 647 Anti-DCAMKL1 antibody [EPR6085]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DCAMKL1 antibody [EPR6085] (AB202755)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
See full target information CLCA1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 8:794 PubMed29075202

2017

Mitochondrial ATP Depletion Disrupts Caco-2 Monolayer Integrity and Internalizes Claudin 7.

Applications

Unspecified application

Species

Unspecified reactive species

Lonneke M JanssenDuijghuijsen,Sander Grefte,Vincent C J de Boer,Lara Zeper,Dorien A M van Dartel,Inge van der Stelt,Melissa Bekkenkamp-Grovenstein,Klaske van Norren,Harry J Wichers,Jaap Keijer
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com