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AB203694

HRP Anti-CLPX antibody [EP8772]

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(2 Publications)

Rabbit Recombinant Monoclonal CLPX antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CLPX

1 Images
Western blot - HRP Anti-CLPX antibody [EP8772] (AB203694)
  • WB

Lab

Western blot - HRP Anti-CLPX antibody [EP8772] (AB203694)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203694 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CLPX antibody [EP8772] (ab203694) at 1/5000 dilution

Lane 1:

HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 69 kDa

Observed band size: 69 kDa

true

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP8772

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent specificity component of the Clp protease complex. Hydrolyzes ATP (PubMed : 28874591). Targets specific substrates for degradation by the Clp complex (PubMed : 11923310, PubMed : 22710082). Can perform chaperone functions in the absence of CLPP. Enhances the DNA-binding activity of TFAM and is required for maintaining a normal mitochondrial nucleoid structure (PubMed : 22841477). ATP-dependent unfoldase that stimulates the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis (PubMed : 28874591). Important for efficient erythropoiesis through up-regulation of heme biosynthesis (PubMed : 25957689, PubMed : 28874591).
See full target information CLPX
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 28:1881-1895 PubMed35417530

2022

Induction of Synthetic Lethality by Activation of Mitochondrial ClpP and Inhibition of HDAC1/2 in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Trang T T Nguyen,Enyuan Shang,Salveena Schiffgens,Consuelo Torrini,Chang Shu,Hasan Orhan Akman,Varun V Prabhu,Joshua E Allen,Mike-Andrew Westhoff,Georg Karpel-Massler,Markus D Siegelin

Frontiers in genetics 10:839 PubMed31572446

2019

Identification of a Prognostic Signature Associated With DNA Repair Genes in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hengzi Sun,Dongyan Cao,Xiangwen Ma,Jiaxin Yang,Peng Peng,Mei Yu,Huimei Zhou,Ying Zhang,Lei Li,Xiao Huo,Keng Shen
View all publications
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Product promise

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