Mouse Monoclonal COX IV antibody - conjugated to HRP. Mitochondrion marker. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164), expandable thumbnail](https://content.abcam.com/products/images/hrp-cox-iv-antibody-mabcam33985-mitochondrial-marker-ab62164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91045.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164)")
![Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164), expandable thumbnail](https://content.abcam.com/products/images/hrp-cox-iv-antibody-mabcam33985-mitochondrial-marker-ab62164--western-blot-img86026.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164)")
![Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164), expandable thumbnail](https://content.abcam.com/products/images/hrp-cox-iv-antibody-mabcam33985-mitochondrial-marker-ab62164--western-blot-img149802.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164)")
Publications
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- The EMBO journal 39:e1039122020Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Lobo-Jarne et. al.PubMed 32511785
- JCI insight 4:2019Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargaret M Lowe et. al.PubMed 31852848
- Biochimica et biophysica acta. Molecular and cell 1863:447-4572018Cardiolipin synthesizing enzymes form a complex that interacts with cardiolipin-dependent membrane organizing proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMauro Serricchio et. al.PubMed 29343430
- Oncotarget 7:77457-774672016Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.Applications:WBReactive species:HumanKaijun Di,Naomi Lomeli,Spencer D Wood,Christopher D Vanderwal,Daniela A BotaPubMed 27764809
- Investigative ophthalmology & visual science 52:4369-802011Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKate A Powell,Jennifer R Davies,Elaine Taylor,Michael A Wride,Marcela VotrubaPubMed 21613372
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Species Human | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Chimpanzee, Zebrafish, Xenopus laevis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Chimpanzee, Zebrafish, Xenopus laevis | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
1 product for Alternative Version
Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Alternative names
COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1
Recommended products
Mouse Monoclonal COX IV antibody - conjugated to HRP. Mitochondrion marker. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- mAbcam33985
- Purity
- IgG fraction
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.
- Biological function summary
COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.
- Pathways
COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.
- Associated diseases and disorders
COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164), expandable thumbnail](https://content.abcam.com/products/images/hrp-cox-iv-antibody-mabcam33985-mitochondrial-marker-ab62164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91045.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab62164 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
![Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164), expandable thumbnail](https://content.abcam.com/products/images/hrp-cox-iv-antibody-mabcam33985-mitochondrial-marker-ab62164--western-blot-img86026.jpg "Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)")
Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab62164 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/5000 dilution
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: Skeletal Muscle (Mouse) Tissue Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 15 kDa
Exposure time: 2min
![Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164), expandable thumbnail](https://content.abcam.com/products/images/hrp-cox-iv-antibody-mabcam33985-mitochondrial-marker-ab62164--western-blot-img149802.jpg "Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)")
Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
All lanes: Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/4000 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 17 kDa
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