HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164)

IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND^TM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab62164 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• WB

Lab

Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab62164 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Predicted band size: 19 kDa

Observed band size: 15 kDa

true

Exposure time: 2min

• WB

Project

Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB62164)

All lanes:

Western blot - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/4000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Predicted band size: 19 kDa

Observed band size: 17 kDa

false