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Mouse Monoclonal CPT1A antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 2 publications.

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Images

Western blot - HRP Anti-CPT1A antibody [8F6AE9] (AB198494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CPT1A antibody [8F6AE9] (AB198494), expandable thumbnail
  • Western blot - HRP Anti-CPT1A antibody [8F6AE9] (AB198494), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Conjugation
HRP
Storage buffer

pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

1 product for Alternative Version

Target data

Function

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed:11350182, PubMed:14517221, PubMed:16651524, PubMed:9691089). Possesses also a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity (PubMed:29425493). Plays an important role in hepatic triglyceride metabolism (By similarity). Plays also a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression (By similarity). Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation (PubMed:38016475). Promotes ROS-induced oxidative stress in liver injury via modulation of NFE2L2 and NLRP3-mediated signaling pathways (By similarity).

Alternative names

CPT1, CPT1A, CPT1-L, Carnitine palmitoyltransferase 1A, Succinyltransferase CPT1A, CPT I, CPTI-L

Recommended products

Mouse Monoclonal CPT1A antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG2b
Conjugation
HRP
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
8F6AE9
Purification technique
Affinity purification
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.

Biological function summary

CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.

Pathways

CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.

Associated diseases and disorders

CPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.

Product promise

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3 product images

  • Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494), expandable thumbnail

    Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)

    Lanes 1 - 4: Merged signal (red and green). Green - ab198494 observed at 88 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab198494 was shown to specifically react with in wild-type HAP1 cells as signal was lost in CPT1A knockout cells. Wild-type and CPT1A knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab198494 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    All lanes: Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494) at 1/5000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: CPT1A knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: MCF7 whole cell lysate at 20 µg

    Predicted band size: 88 kDa

    Observed band size: 88 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CPT1A antibody [8F6AE9] (ab198494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)

    IHC image of CPT1A staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198494 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494), expandable thumbnail

    Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198494 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494) at 1/5000 dilution

    Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: Heart (Human) Tissue Lysate - adult normal tissue at 20 µg

    Lane 3: Liver (Rat) Tissue Lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 88 kDa

    Observed band size: 88 kDa

    Exposure time: 1min

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Product protocols

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