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AB199992

HRP Anti-CRALBP antibody [B2]

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(1 Publication)

Mouse Monoclonal CRALBP antibody - conjugated to HRP. Suitable for WB and reacts with Rat samples. Cited in 1 publication.

View Alternative Names

CRALBP, RLBP1, Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein

1 Images
Western blot - HRP Anti-CRALBP antibody [B2] (AB199992)
  • WB

Lab

Western blot - HRP Anti-CRALBP antibody [B2] (AB199992)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199992 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CRALBP antibody [B2] (ab199992) at 1/5000 dilution

All lanes:

Eye (Rat) Tissue Lysate at 10 µg

Predicted band size: 36 kDa

Observed band size: 36 kDa

true

Exposure time: 3min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B2

Isotype

IgG2a

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'CRALBP' also known as cellular retinaldehyde-binding protein is a protein involved in the visual cycle. It weighs approximately 36 kDa. Expressed mainly in the retina CRALBP is found in retinal pigment epithelial (RPE) cells and Muller glial cells. It binds to retinoids which are critical for proper vision functioning. CRALBP also goes by the synonyms such as 'BSA b 2' which is used in specific contexts related to its role in retinal metabolism.
Biological function summary

The protein aids in the effective conversion of all-trans-retinal to 11-cis-retinal an important step in the visual cycle. It acts as a chaperone that stabilizes retinoid intermediates facilitating their transport between various retinal cell types. CRALBP forms part of a complex with retinoid isomerohydrolase and 11-cis-retinol dehydrogenase enhancing its binding and transport function. This ensures smooth retinoid flux necessary for regenerating the visual pigment rhodopsin.

Pathways

CRALBP is integral to two main pathways; the retinoid cycle in cones and the rhodopsin regeneration pathway in rods. In these pathways it interacts with proteins such as RPE65 and 11-cis-retinol dehydrogenase ensuring efficient retinoid transfer and isomerization. This important involvement supports the regeneration of visual pigments necessary for photoreceptor cell function under various lighting conditions.

CRALBP associates with retina-related conditions notably congenital stationary night blindness (CSNB) and retinitis pigmentosa (RP). Mutations affecting CRALBP can alter retinoid transport and contribute to these disorders. Additionally CRALBP dysfunction links to other proteins like RPE65 and RDH5 which can also play roles in the pathogenesis of these visual impairments. Understanding these relationships helps in developing targeted treatments for retinoid-related visual disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
See full target information RLBP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:145 PubMed40713709

2025

Effect of Wendan decoction granules on preventing endolymphatic hydrops and protecting vestibular function in Guinea pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Xia,Tiancheng Xie,Jinli Liu,Weikang Cheng,Longyan Liao,Ming Chen,Haibing Hua,Xiaobing Hou
View all publications
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Product promise

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