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AB202580

HRP Anti-CRISPR-Cas9 antibody [7A9-3A3]

4

(1 Review)

|

(5 Publications)

Mouse Monoclonal H2B1L antibody - conjugated to HRP. Suitable for WB and reacts with Transfected cell lysate - Streptococcus pyogenes samples. Cited in 5 publications.

View Alternative Names

H2BFC, HIST1H2BL, H2BC13, Histone H2B type 1-L, Histone H2B.c, H2B/c

1 Images
Western blot - HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (AB202580)
  • WB

Lab

Western blot - HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (AB202580)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202580 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (ab202580) at 1/5000 dilution

Lane 1:

Cas 9 negative control (S2 non-transfected cell lysate) at 20 µg

Lane 2:

Cas 9 transfected cells (S2 cells transfected with CRISPR-Cas9 lysate) at 20 µg

Predicted band size: 160 kDa

Observed band size: 180 kDa

true

Exposure time: 2min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7A9-3A3

Isotype

IgG1

Light chain type

kappa

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Streptococcus pyogenes": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Streptococcus pyogenes": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>We recommend using 3% milk as the blocking agent for Western blot.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.
Biological function summary

The Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.

Pathways

CRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.

CRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H2BC13
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:914 PubMed34615851

2021

Inhibition of the ubiquitin-proteasome system by an NQO1-activatable compound.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana A Giovannucci,Florian A Salomons,Martin Haraldsson,Lotta H M Elfman,Malin Wickström,Patrick Young,Thomas Lundbäck,Jürgen Eirich,Mikael Altun,Rozbeh Jafari,Anna-Lena Gustavsson,John Inge Johnsen,Nico P Dantuma

PLoS genetics 16:e1008924 PubMed32673314

2020

Resection and repair of a Cas9 double-strand break at CTG trinucleotide repeats induces local and extensive chromosomal deletions.

Applications

Unspecified application

Species

Unspecified reactive species

Valentine Mosbach,David Viterbo,Stéphane Descorps-Declère,Lucie Poggi,Wilhelm Vaysse-Zinkhöfer,Guy-Franck Richard

PloS one 14:e0222588 PubMed31553754

2019

CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein.

Applications

Unspecified application

Species

Unspecified reactive species

Milad Babaei,Yuekun Liu,Shelly M Wuerzberger-Davis,Ethan Z McCaslin,Christopher J DiRusso,Alan T Yeo,Larisa Kagermazova,Shigeki Miyamoto,Thomas D Gilmore

Nucleic acids research 46:11115-11125 PubMed30289463

2018

Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyi Zhang,Christopher A Voigt

Nature protocols 13:1465-1487 PubMed29844520

2018

Generation and validation of homozygous fluorescent knock-in cells using CRISPR-Cas9 genome editing.

Applications

Unspecified application

Species

Unspecified reactive species

Birgit Koch,Bianca Nijmeijer,Moritz Kueblbeck,Yin Cai,Nike Walther,Jan Ellenberg
View all publications
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