HRP Anti-CSN8 antibody [EPR5139]

• WB

Lab

Western blot - HRP Anti-CSN8 antibody [EPR5139] (AB208842)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208842 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CSN8 antibody [EPR5139] (ab208842) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 2:

Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 3:

SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 23 kDa

Observed band size: 21 kDa

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Exposure time: 12min