Rabbit Recombinant Monoclonal CTCF antibody - conjugated to HRP.
IgG
Rabbit
HRP
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Select an associated product type
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (PubMed:26321640).
Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog, CTCF
Rabbit Recombinant Monoclonal CTCF antibody - conjugated to HRP.
Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog, CTCF
IgG
Rabbit
HRP
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR7314(B)
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
This conjugated primary antibody is “made to order” and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
CTCF also known as the CCCTC-binding factor is a highly conserved zinc finger protein involved in transcriptional regulation and chromatin organization. It has a molecular weight of approximately 82 kDa. The CTCF protein plays a critical mechanical role in controlling the three-dimensional architecture of the genome by binding to specific DNA sequences and forming chromatin loops. It is widely expressed in various cell types across tissues where it acts as a transcriptional repressor and activator depending on the context. CTCF immunofluorescence techniques enable the visualization of its dynamic distribution and expression within the nucleus.
The multipurpose CTCF protein acts in coordinating the spatial organization of the genome. It functions as an insulator by regulating the boundaries between different chromosomal domains and controlling gene expression. CTCF operates within various complexes interacting with cohesin a vital protein complex that facilitates loop formation and influences genome architecture. This interaction helps in maintaining the integrity of the genome structure and proper chromatin insulation which are essential for normal gene function.
CTCF plays significant roles in epigenetic regulatory networks and transcriptional pathways. In the epigenetic landscape it influences gene expression through modulation of DNA methylation states at CpG islands interacting with proteins like DNA methyltransferases. In transcriptional pathways CTCF interacts with nuclear factor Y (NF-Y) which contributes to cell cycle regulation by modulating the expression of cell cycle genes. These pathways reflect CTCF's versatility in gene regulation and its influence on maintaining cellular homeostasis.
CTCF disruptions have been implicated in cancer and intellectual disabilities. Mutations or altered expression of CTCF can lead to tumorigenesis as CTCF acts as a tumor suppressor by controlling oncogene and tumor suppressor gene expression. In intellectual disabilities CTCF mutations affect brain development by disrupting the expression of neuronal genes. The protein's interaction with cohesin has links to disorders such as Cornelia de Lange syndrome where cohesin complex dysfunction parallels the phenotypes seen with CTCF aberrations.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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