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AB106490

HRP Anti-CTLA4 antibody [KT50]

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(1 Publication)

Mouse Monoclonal CTLA4 antibody - conjugated to HRP. Suitable for sELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CTLA4.

View Alternative Names

CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4

1 Images
Sandwich ELISA - HRP Anti-CTLA4 antibody [KT50] (AB106490)
  • sELISA

Unknown

Sandwich ELISA - HRP Anti-CTLA4 antibody [KT50] (AB106490)

ab106490 at 1/1000 dilution detecting CTLA4 by sandwich ELISA. The capture antibody is a mouse monoclonal (IgG1) raised against Human CTLA4.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KT50

Isotype

IgG2a

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CTLA4.

P16410

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1/1000", "sELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTLA-4 also known as CD152 is an immune checkpoint receptor with a molecular weight of approximately 34 kDa. This protein is expressed on the surface of T-cells especially after activation and sometimes on regulatory T-cells (Tregs). CTLA-4 competes with CD28 for binding to the same ligands CD80 and CD86 on antigen-presenting cells. Unlike CD28 which stimulates T-cell activation CTLA-4 sends inhibitory signals to downregulate immune responses. By doing this CTLA-4 helps maintain immune system homeostasis and prevents autoimmune reactions.
Biological function summary

The CTLA-4 protein functions as a critical regulator of the immune system. It interacts with CD80/CD86 in a complex that effectively transmits inhibitory signals to T-cells. CTLA-4 controls the amplitude of the initial activation of T-cells ensuring that the body's immune responses are kept under control. In regulatory T-cells CTLA-4 engagement contributes to their immunosuppressive functions through which they maintain tolerance to self-antigens and prevent overactive immune reactions.

Pathways

CTLA-4 involves itself in the adaptive immune response pathways specifically the regulation of T-cell activity. CTLA-4's interaction with CD80/CD86 modulates pathways associated with TCR (T-cell receptor) signaling. When CTLA-4 binds its ligands it recruits phosphatases such as SHP-2 that dephosphorylate signaling proteins leading to the downregulation of T-cell interaction. This pathway interaction exemplifies how CTLA-4 acts in concert with proteins like CD28 to finely tune immune responses.

CTLA-4 plays a role in autoimmune diseases and cancer. The dysregulation of CTLA-4 function can lead to autoimmune disorders where the immune system attacks the body's own tissue. In cancer tumor cells may manipulate CTLA-4 pathways to evade immune surveillance. Targeted therapies like anti-CTLA-4 antibodies such as ipilimumab have been developed to block CTLA-4 aiming to enhance the immune system’s ability to attack cancer cells. These treatments highlight the role of CTLA-4 in balancing immune activity and the potential to alter this balance for therapeutic benefit.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
See full target information CTLA4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:26651-62 PubMed26450852

2015

Liver X receptor ligand cytotoxicity in colon cancer cells and not in normal colon epithelial cells depends on LXRβ subcellular localization.

Applications

Unspecified application

Species

Unspecified reactive species

Flavie Courtaut,Valentin Derangère,Angélique Chevriaux,Sylvain Ladoire,Alexia K Cotte,Laurent Arnould,Romain Boidot,Mickaël Rialland,François Ghiringhelli,Cédric Rébé
View all publications
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