Rabbit Recombinant Monoclonal Cyclin B1 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.
Images
![Western blot - HRP Anti-Cyclin B1 antibody [Y106] (AB193977), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-b1-antibody-y106-ab193977--western-blot-img97954.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Cyclin B1 antibody [Y106] (AB193977)")
![Western blot - HRP Anti-Cyclin B1 antibody [Y106] (AB193977), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-b1-antibody-y106-ab193977--western-blot-img97492.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Cyclin B1 antibody [Y106] (AB193977)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Not recommended |
| Rat | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
7 products for Alternative Product
Target data
Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Alternative names
G2/mitotic-specific cyclin-B1, CCNB, CCNB1
Recommended products
Rabbit Recombinant Monoclonal Cyclin B1 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples.
Alternative names
G2/mitotic-specific cyclin-B1, CCNB, CCNB1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
Y106
- Purification technique
Affinity purification Protein A
- Epitope
ab32053 reacts with an epitope located in teh C terminal region of Cyclin B1.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-b1-antibody-y106-ab193977--western-blot-img97954.jpg "Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)")
Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)
ab193977 was shown to specifically react with Cyclin B1 in wild-type HAP1 cells as signal was lost in CCNB1 (Cyclin B1) knockout cells. Wild-type and CCNB1 (Cyclin B1) knockout samples were subjected to SDS-PAGE. ab193977 and Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
All lanes: Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977) at 1/5000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: CCNB1 (Cyclin B1) knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 48 kDa
Observed band size: 51 kDa
Exposure time: 1min
![Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-b1-antibody-y106-ab193977--western-blot-img97492.jpg "Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)")
Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193977 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Cyclin B1 antibody [Y106] (ab193977) at 1/5000 dilution
Lane 1: Western blot - HeLa whole cell lysate (HeLa whole cell lysate ab150035) at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 58 kDa
Exposure time: 1min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com