Rabbit Monoclonal Cyclin D1 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Images
![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-d1-antibody-epr2241-ab190564--western-blot-img95061.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)")
![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-d1-antibody-epr2241-ab190564--western-blot-img73606.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)")
![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-d1-antibody-epr2241-ab190564--western-blot-img32023.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)")
Publications
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- Frontiers in aging neuroscience 13:6334952021Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong-Cong Qi,Xing-Xing Chen,Xin-Ran Gao,Jing-Xian Xu,Sen Liu,Jin-Fang GePubMed 33732137
- International journal of molecular medicine 46:1135-11452020Propofol‑induced HOXA11‑AS promotes proliferation, migration and invasion, but inhibits apoptosis in hepatocellular carcinoma cells by targeting miR‑4458.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFurong Song et. al.PubMed 32705160
- Cell reports 30:2237-2247.e62020OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLien Verboom,Arne Martens,Dario Priem,Esther Hoste,Mozes Sze,Hanna Vikkula,Lisette Van Hove,Sofie Voet,Jana Roels,Jonathan Maelfait,Laura Bongiovanni,Alain de Bruin,Charlotte L Scott,Yvan Saeys,Manolis Pasparakis,Mathieu J M Bertrand,Geert van LooPubMed 32075762
- Communications biology 2:3072019Targeting p16-induced senescence prevents cigarette smoke-induced emphysema by promoting IGF1/Akt1 signaling in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher T Cottage et. al.PubMed 31428695
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes - |
Species Rat | Dilution info 1/5000 | Notes - |
Species Human | Dilution info 1/5000 | Notes - |
Associated Products
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Target data
Function
The protein expressed by the CCND1 gene acts as a regulatory component of the cyclin D1-CDK4 (DC) complex, which phosphorylates and inhibits retinoblastoma (RB) protein family members, including RB1, regulating the cell cycle during the G(1)/S transition. Phosphorylation of RB1 allows the transcription factor E2F to dissociate from the RB/E2F complex, enabling transcription of E2F target genes responsible for G(1) phase progression. In early G(1) phase, it hypophosphorylates RB1. Cyclin D-CDK4 complexes integrate various mitogenic and antimitogenic signals. The protein also serves as a substrate for SMAD3, phosphorylating it in a cell-cycle-dependent manner to repress its transcriptional activity, and forms part of the cyclin D1/CDK4/CDKN1B complex necessary for nuclear translocation and activity. Additionally, it exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters, independent of the cell cycle. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
BCL1, PRAD1, CCND1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1
Recommended products
Rabbit Monoclonal Cyclin D1 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2241
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.4 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-d1-antibody-epr2241-ab190564--western-blot-img95061.jpg "Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)")
Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk blocking buffer before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution
Lane 1: MCF7 Whole Cell Lysate at 10 µg
Lane 2: RAW 264.7 Whole Cell Lysate at 10 µg
Lane 3: PC12 Whole cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 4min
![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-d1-antibody-epr2241-ab190564--western-blot-img73606.jpg "Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)")
Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1min
![Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564), expandable thumbnail](https://content.abcam.com/products/images/hrp-cyclin-d1-antibody-epr2241-ab190564--western-blot-img32023.jpg "Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)")
Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution
All lanes: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1min
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