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AB190564

HRP Anti-Cyclin D1 antibody [EPR2241]

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(4 Publications)

Rabbit Recombinant Monoclonal Cyclin D1 antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

BCL1, PRAD1, CCND1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1

3 Images
Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)
  • WB

Lab

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk blocking buffer before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution

Lane 1:

MCF7 Whole Cell Lysate at 10 µg

Lane 2:

RAW 264.7 Whole Cell Lysate at 10 µg

Lane 3:

PC12 Whole cell Lysate at 10 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 4min

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)
  • WB

Lab

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 1min

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)
  • WB

Lab

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution

All lanes:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2241

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Mouse, Rat, Human, Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 33854235, PubMed : 8114739, PubMed : 8302605). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8114739, PubMed : 8302605). Hypophosphorylates RB1 in early G(1) phase (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8114739, PubMed : 8302605). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8302605). Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed : 15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed : 16569215, PubMed : 18417529).
See full target information CCND1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 13:633495 PubMed33732137

2021

Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Cong Qi,Xing-Xing Chen,Xin-Ran Gao,Jing-Xian Xu,Sen Liu,Jin-Fang Ge

International journal of molecular medicine 46:1135-1145 PubMed32705160

2020

Propofol‑induced HOXA11‑AS promotes proliferation, migration and invasion, but inhibits apoptosis in hepatocellular carcinoma cells by targeting miR‑4458.

Applications

Unspecified application

Species

Unspecified reactive species

Furong Song,Jun Liu,Yawei Feng,Yi Jin

Cell reports 30:2237-2247.e6 PubMed32075762

2020

OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lien Verboom,Arne Martens,Dario Priem,Esther Hoste,Mozes Sze,Hanna Vikkula,Lisette Van Hove,Sofie Voet,Jana Roels,Jonathan Maelfait,Laura Bongiovanni,Alain de Bruin,Charlotte L Scott,Yvan Saeys,Manolis Pasparakis,Mathieu J M Bertrand,Geert van Loo

Communications biology 2:307 PubMed31428695

2019

Targeting p16-induced senescence prevents cigarette smoke-induced emphysema by promoting IGF1/Akt1 signaling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher T Cottage,Norman Peterson,Jennifer Kearley,Aaron Berlin,Ximing Xiong,Anna Huntley,Weiguang Zhao,Charles Brown,Annik Migneault,Kamelia Zerrouki,Gerald Criner,Roland Kolbeck,Jane Connor,Raphael Lemaire
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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