AB200425

HRP Anti-Cyclin E2 antibody [EP454Y]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cyclin E2 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

G1/S-specific cyclin-E2, CCNE2

1 Images

Lab

Western blot - HRP Anti-Cyclin E2 antibody [EP454Y] (AB200425)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200425 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin E2 antibody [EP454Y] (ab200425) at 1/5000 dilution

All lanes:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Exposure time: 20min

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cyclin E2 antibody [EP454Y]
660 APC

APC Anti-Cyclin E2 antibody [EP454Y]
578 PE

PE Anti-Cyclin E2 antibody [EP454Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cyclin E2 antibody [EP454Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cyclin E2 antibody [EP454Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Cyclin E2 antibody [EP454Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP454Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the control of the cell cycle at the late G1 and early S phase.

See full target information Cyclin E2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 20:202 PubMed32514247

2020

Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma cell growth via sponging miR-577.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Sun,Rui Yu,Chunfeng Wang,Jianning Yao,Lianfeng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Cyclin E2 antibody [EP454Y]

Western blot - HRP Anti-Cyclin E2 antibody [EP454Y] (AB200425)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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