HRP Anti-Cytochrome C antibody [EPR1327]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cytochrome C antibody [EPR1327] (AB193239)

IHC image of Cytochrome C staining in a section of formalin-fixed paraffin-embedded normal Human kidney tissue. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab193239 at a working dilution of 1 in 10000 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• WB

Lab

Western blot - HRP Anti-Cytochrome C antibody [EPR1327] (AB193239)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193239 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cytochrome C antibody [EPR1327] (ab193239) at 1/2000 dilution

Lane 1:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Heart (Human) Tissue Lysate - adult normal tissue at 10 µg

Predicted band size: 11 kDa

Observed band size: 14 kDa

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Exposure time: 20min