AB205484

HRP Anti-DDB2 antibody [EPR9811]

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DDB2 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DNA damage-binding protein 2, DDB p48 subunit, Damage-specific DNA-binding protein 2, UV-damaged DNA-binding protein 2, DDBb, UV-DDB 2, DDB2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-DDB2 antibody [EPR9811] (AB205484)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-DDB2 antibody [EPR9811] (AB205484)

IHC image of DDB2 staining in a section of formalin-fixed paraffin-embedded normal human skin*, performed on a Leica BOND^TM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution

for 20mins. The section was then incubated with ab205484, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Lab

Western blot - HRP Anti-DDB2 antibody [EPR9811] (AB205484)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205484 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-DDB2 antibody [EPR9811] (ab205484) at 1/2000 dilution

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 48 kDa

Observed band size: 45 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9811

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDB2 also known as DNA damage-binding protein 2 plays a mechanical role in the nucleotide excision repair (NER) pathway. It is a part of the UV-damaged DNA-binding protein complex and assists in recognizing and cutting damaged DNA. The mass of DDB2 is approximately 48 kDa. The expression of DDB2 occurs broadly but it shows enhanced expression in tissues exposed to UV light like skin.

Biological function summary

Engagement in DNA repair mechanisms allows DDB2 to help maintain genomic stability. It forms a critical part of the DDB1-DDB2 complex which collaborates with other proteins in the initial damage recognition step of NER. This involvement ensures the repair of UV-induced damage and bulky DNA adducts preventing mutations that can cause harmful genetic alterations.

Pathways

The involvement of DDB2 is important within the nucleotide excision repair and transcription-coupled repair pathways. In these pathways DDB2 partners with proteins such as XPC and XPA facilitating the recognition and verification of DNA damage. These interactions ensure an accurate and efficient repair process protecting cells from potential genotoxic stress.

DDB2 has relevance to skin cancer and xeroderma pigmentosum. Among these mutations or dysregulation involving DDB2 can lead to skin cancer due to impaired DNA repair response to UV damage. It connects to p53 a protein that regulates the cell cycle and likewise contributes to cellular responses against DNA damage. In xeroderma pigmentosum defects in components of the NER pathway including DDB2 lead to extreme sensitivity to sunlight and subsequent skin abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed : 10882109, PubMed : 11278856, PubMed : 11705987, PubMed : 12732143, PubMed : 15882621, PubMed : 16473935, PubMed : 18593899, PubMed : 32789493, PubMed : 9892649). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (PubMed : 10882109, PubMed : 11278856, PubMed : 11705987, PubMed : 12944386, PubMed : 14751237, PubMed : 16260596, PubMed : 32789493). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (PubMed : 10882109, PubMed : 11278856, PubMed : 11705987, PubMed : 12944386, PubMed : 16260596). Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1) (PubMed : 12732143, PubMed : 15882621, PubMed : 16473935, PubMed : 18593899, PubMed : 26572825). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage (PubMed : 16473935, PubMed : 16678110). The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair (PubMed : 16473935, PubMed : 16678110). The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER (PubMed : 15882621). The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation : recognizes KAT7/HBO1 following phosphorylation by ATR (PubMed : 26572825).. Isoform D1. Inhibits UV-damaged DNA repair.. Isoform D2. Inhibits UV-damaged DNA repair.

See full target information DDB2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110760 PubMed39286490

2024

Efficient proximal tubule-on-chip model from hiPSC-derived kidney organoids for functional analysis of renal transporters.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Ma,Ramin Banan Sadeghian,Ryosuke Negoro,Kazuya Fujimoto,Toshikazu Araoka,Naoki Ishiguro,Minoru Takasato,Ryuji Yokokawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-DDB2 antibody [EPR9811]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-DDB2 antibody [EPR9811] (AB205484)

Western blot - HRP Anti-DDB2 antibody [EPR9811] (AB205484)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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