AB2493

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Name: Anti-DDDDK tag (Binds to FLAG® tag sequence) HRP antibody (ab2493) | Abcam
Brand: Abcam Limited
SKU: AB2493
Price: 430 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to HRP. Suitable for ELISA, WB and reacts with Tag samples. Cited in 4 publications.

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

1 Images

ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB2493)

ELISA

Supplier Data

ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB2493)

Antigen concentration : 1000 ng/well. ab2493 was incubated for 60 minutes. Developed with TMB. Microplate reader set at 450nm.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 19:378-388 PubMed36782012

2023

TMEM164 is an acyltransferase that forms ferroptotic C20:4 ether phospholipids.

Applications

Unspecified application

Species

Unspecified reactive species

Alex Reed,Timothy Ware,Haoxin Li,J Fernando Bazan,Benjamin F Cravatt

Infection and immunity 88: PubMed32817331

2020

Single Amino Acid Replacements in RocA Disrupt Protein-Protein Interactions To Alter the Molecular Pathogenesis of Group A .

Applications

Unspecified application

Species

Unspecified reactive species

Paul E Bernard,Amey Duarte,Mikhail Bogdanov,James M Musser,Randall J Olsen

Nucleic acids research 46:6401-6415 PubMed29961805

2018

Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan A Belashov,David W Crawford,Chapin E Cavender,Peng Dai,Patrick C Beardslee,David H Mathews,Bradley L Pentelute,Brian R McNaughton,Joseph E Wedekind

Nucleic acids research 46:7250-7260 PubMed29945249

2018

Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Kling,Thomas Spaller,Jana Schiefner,Doreen Bönisch,Thomas Winckler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (AB2493)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (4)

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