Rabbit Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 1 publication.
Images
![Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245895), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-ab245895--western-blot-img129815.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245895)")
Publications
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- FASEB bioAdvances 4:180-1962022Critical cysteines in the functional interaction of adenylyl cyclase isoform 6 with Gαs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnjali Y Bhagirath,Vikram Bhatia,Manoj Reddy Medapati,Nisha Singh,Martha Hinton,Prashen Chelikani,Shyamala DakshinamurtiPubMed 35664968
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Tag | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Rabbit Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20018-251
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-ab245895--western-blot-img129815.jpg "Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895)")
Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895)
ab245895 was shown to react with DDDDK tag in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab245895 overnight at 4°C at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged.
All lanes: Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895) at 1/1000 dilution
Lane 1: Recombinant human RANKL protein (Active) (Recombinant human RANKL protein (Active) ab157289) cell lysate at 0.5 µg
Lane 2: Recombinant human RANKL protein (Active) (Recombinant human RANKL protein (Active) ab157289) cell lysate at 0.1 µg
Lane 3: Recombinant human TRAIL protein (Recombinant human TRAIL protein ab157345) cell lysate at 0.5 µg
Lane 4: Recombinant human TRAIL protein (Recombinant human TRAIL protein ab157345) cell lysate at 0.1 µg
Performed under reducing conditions.
Observed band size: 35 kDa
Exposure time: 10s
Downloads
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