HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

• WB

Lab

Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245895)

ab245895 was shown to react with DDDDK tag in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab245895 overnight at 4°C at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.

All lanes:

Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895) at 1/1000 dilution

Lane 1:

Recombinant human RANKL protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-rankl-protein-active-ab157289'>ab157289</a>) cell lysate at 0.5 µg

Lane 2:

Recombinant human RANKL protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-rankl-protein-active-ab157289'>ab157289</a>) cell lysate at 0.1 µg

Lane 3:

Recombinant human TRAIL protein (<a href='/en-us/products/proteins-peptides/recombinant-human-trail-protein-ab157345'>ab157345</a>) cell lysate at 0.5 µg

Lane 4:

Recombinant human TRAIL protein (<a href='/en-us/products/proteins-peptides/recombinant-human-trail-protein-ab157345'>ab157345</a>) cell lysate at 0.1 µg

Observed band size: 35 kDa

false

Exposure time: 10s