Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] is a mouse monoclonal HRP conjugated antibody that is used to detect DDDDK tag (Binds to FLAG® tag sequence) in ELISA, Western blot.
- Cited in over 85 publications
- Binds to the FLAG® epitope tag sequence
Images
![Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763--western-blot-img24328.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763)")
![ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763--elisa-img53108.jpg/_jcr_content/renditions/webp-475.webp "ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763)")
![Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763--western-blot-img52382.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763)")
Publications
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- Signal transduction and targeted therapy 8:2292023Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoyuan Ma,Anji Ju,Shaosen Zhang,Qi An,Siran Xu,Jie Liu,Li Yu,Yan Fu,Yongzhang LuoPubMed 37321990
- Nature communications 14:20452023Lung-specific MCEMP1 functions as an adaptor for KIT to promote SCF-mediated mast cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoun Jung Choi,Ji-Seung Yoo,Kyle Jung,Logan Rice,Dokyun Kim,Violetta Zlojutro,Matthew Frimel,Evan Madden,Un Yung Choi,Suan-Sin Foo,Younho Choi,Zhongyi Jiang,Holly Johnson,Mi-Jeong Kwak,Seokmin Kang,Brian Hong,Gil Ju Seo,Stephanie Kim,Shin-Ae Lee,Samad Amini-Bavil-Olyaee,Hadi Maazi,Omid Akbari,Kewal Asosingh,Jae U JungPubMed 37041174
- Biology 12:2023Characterization of Adenylyl Cyclase Isoform 6 Residues Interacting with Forskolin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVikram Bhatia,Saeid Maghsoudi,Martha Hinton,Anjali Y Bhagirath,Nisha Singh,Appalaraju Jaggupilli,Prashen Chelikani,Shyamala DakshinamurtiPubMed 37106773
- Clinical and translational medicine 13:e10072023In-frame deletion of SMC5 related with the phenotype of primordial dwarfism, chromosomal instability and insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjiao Zhu,Yuanping Shi,Changrun Zhang,Yajie Peng,Yueyue Wan,Yue Xu,Xuemeng Liu,Bing Han,Shuangxia Zhao,Yanping Kuang,Huaidong Song,Jie QiaoPubMed 36627765
- Cell communication and signaling : CCS 20:1642022RBCK1 is an endogenous inhibitor for triple negative breast cancer via hippo/YAP axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongbo Li,Peng Su,Yinlu Ding,Honglei Gao,Huijie Yang,Xin Li,Xiao Yang,Yan Xia,Chenmiao Zhang,Mingxi Fu,Dehai Wang,Ye Zhang,Shu Zhuo,Jian Zhu,Ting ZhuangPubMed 36280829
- Cell death & disease 13:7092022DNA damage-induced paraspeckle formation enhances DNA repair and tumor radioresistance by recruiting ribosomal protein P0.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Long Wang,Wan-Wen Zhao,Shao-Mei Bai,Yan Ma,Xin-Ke Yin,Li-Li Feng,Guang-Dong Zeng,Fang Wang,Wei-Xing Feng,Jian Zheng,Ying-Nai Wang,Bing Zeng,Quentin Liu,Mien-Chie Hung,Xiang-Bo WanPubMed 35974014
- Nature 609:785-7922022Coronaviruses exploit a host cysteine-aspartic protease for replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHin Chu,Yuxin Hou,Dong Yang,Lei Wen,Huiping Shuai,Chaemin Yoon,Jialu Shi,Yue Chai,Terrence Tsz-Tai Yuen,Bingjie Hu,Cun Li,Xiaoyu Zhao,Yixin Wang,Xiner Huang,Kin Shing Lee,Cuiting Luo,Jian-Piao Cai,Vincent Kwok-Man Poon,Chris Chung-Sing Chan,Anna Jinxia Zhang,Shuofeng Yuan,Ko-Yung Sit,Dominic Chi-Chung Foo,Wing-Kuk Au,Kenneth Kak-Yuen Wong,Jie Zhou,Kin-Hang Kok,Dong-Yan Jin,Jasper Fuk-Woo Chan,Kwok-Yung YuenPubMed 35922005
- Proceedings of the National Academy of Sciences of the United States of America 119:e22045391192022SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhang Xu,Jung-Hyun Choi,David L Dai,Jun Luo,Reese Jalal Ladak,Qian Li,Yimeng Wang,Christine Zhang,Shane Wiebe,Alex C H Liu,Xiaozhuo Ran,Jiaqi Yang,Parisa Naeli,Aitor Garzia,Lele Zhou,Niaz Mahmood,Qiyun Deng,Mohamed Elaish,Rongtuan Lin,Lara K Mahal,Tom C Hobman,Jerry Pelletier,Tommy Alain,Silvia M Vidal,Thomas Duchaine,Mohammad T Mazhab-Jafari,Xiaojuan Mao,Seyed Mehdi Jafarnejad,Nahum SonenbergPubMed 35878012
- Nature communications 13:30752022YAP inhibits ERα and ER breast cancer growth by disrupting a TEAD-ERα signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Li,Shu Zhuo,Ting Zhuang,Yong Suk Cho,Guojin Wu,Yuchen Liu,Kun Mu,Kai Zhang,Peng Su,Yingzi Yang,Cheng Cheng Zhang,Jian Zhu,Jin JiangPubMed 35654829
- STAR protocols 3:1011972022An optimized protocol to assess SUMOylation in the plant using two-component DEX-inducible transformants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Dong,Zhi-Cheng Hu,Lars ØstergaardPubMed 35243380
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 1.5% BSA, 0.58% Sodium chloride- Form
- Lyophilized
- Clonality
- Monoclonal
Consider this alternative
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Tag | Tested | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/2000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/500 - 1/10000 | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
8 products for Alternative Version
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] is a mouse monoclonal HRP conjugated antibody that is used to detect DDDDK tag (Binds to FLAG® tag sequence) in ELISA, Western blot.
- Cited in over 85 publications
- Binds to the FLAG® epitope tag sequence
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- HRP
- Storage buffer
pH: 7.4
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 1.5% BSA, 0.58% Sodium chloride- Form
- Lyophilized
- Clonality
- Monoclonal
- Clone number
- M2
- Purification technique
- Affinity purification Protein A
- Reconstitution
- reconstitute with water at 1mL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, WB.
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) has been cited over 88 times in peer reviewed journals and is trusted by the scientific community.
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) has 8 independent reviews from customers.
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) specifically detects DDDDK tag (Binds to FLAG® tag sequence) (UniProt ID: ; Molecular weight: 1kDa) and is sold in a 100 ug selling size.
This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763--western-blot-img24328.jpg "Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)")
This image is courtesy of an anonymous customer reviewWestern blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.
5% milk blocking was used for 1 hour.
All lanes: Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) at 1/3000 dilution
All lanes: Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate). at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 45s
![ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763--elisa-img53108.jpg "ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)")
This image is courtesy of an anonymous customer reviewELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
ELISA for the detection of FLAG®-tag purified protein from Escherichia coli, using Anti-NQO1 antibody [A180] ab28947 (1/5000). Please refer to abreview for further experimental details.
Please note that in order to show a clearer image, we have brightened the original image.
![Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763), expandable thumbnail](https://content.abcam.com/products/images/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763--western-blot-img52382.jpg "Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)")
This image is courtesy of an anonymous customer reviewWestern blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
Saccharomyces cerevisiae purified FLAG®-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.
All lanes: Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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