AB6212

HRP Anti-Digoxigenin antibody [HRP.21H8]

Name: HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212) | Abcam
Brand: Abcam Limited
SKU: AB6212
Price: 400 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(23 Publications)

Anti-Digoxigenin antibody [.21H8] - HRP conjugated (ab6212) is a mouse monoclonal antibody detecting Digoxigenin in IHC-Fr.

- Over 20 publications
- Trusted since 2002

View Alternative Names

1672-46-4, BRN 0096479, DIG, EINECS 216-806-2, HSDB 7108, Lanadigenin, Lanadigigenin, ST056392

1 Images

Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (AB6212)

IHC-Fr

AbReview3846****

Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (AB6212)

ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.

This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HRP.21H8

Isotype

IgG1

Light chain type

kappa

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

IHC-Fr

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200 - 1/2000", "IHCFr-species-notes": "<p>1/200 - 1/2000. Use in combination with tyramide signal amplification.</p>" } } }

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Product details

What is this antibody validated in?
HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr). Trusted by the scientific community
Anti-Digoxigenin [HRP.21H8] HRP (ab6212) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.

Related products
Antibody clone HRP.21H8 is also available pre-conjugated to a variety of labels for your convenience – Anti-Digoxigenin HRP [HRP.21H8] (ab6212).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Digoxigenin

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:147 PubMed35069828

2022

Role of cyclin-dependent kinase 5 in early brain injury following experimental subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Ding,Liexiang Zhang,Wei Zhou,Hai Lu,Xingde Gao,Jian Li,Jingde Liu,Xiaowang Niu,Jing Zheng

Journal of cellular biochemistry 123:231-247 PubMed34636091

2021

Long noncoding RNA Gas5 induces cell apoptosis and inhibits tumor growth via activating the CHOP-dependent endoplasmic reticulum stress pathway in human hepatoblastoma HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Yi Zhang,Hao-Lian Zhan,Ming-Kai Li,Guan-Di Wu,Zhe Liu,Ling-Fei Wu

Placenta 109:64-71 PubMed33990028

2021

microRNA-210/ Long non-coding RNA MEG3 axis inhibits trophoblast cell migration and invasion by suppressing EMT process.

Applications

Unspecified application

Species

Unspecified reactive species

Rongli Wang,Li Zou,Xinyuan Yang

Aging 13:6752-6764 PubMed33621204

2021

Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yamei Zhang,Junying Liu,Xin Wang,Jinfeng Zhang,Chenchen Xie

Biophysical journal 120:631-641 PubMed33453266

2021

High-throughput mechanobiology: Force modulation of ensemble biochemical and cell-based assays.

Applications

Unspecified application

Species

Unspecified reactive species

Ália Dos Santos,Natalia Fili,David S Pearson,Yukti Hari-Gupta,Christopher P Toseland

Oncology reports 44:2130-2142 PubMed32901893

2020

Long non‑coding RNA MEG3 suppresses epithelial‑to‑mesenchymal transition by inhibiting the PSAT1‑dependent GSK‑3β/Snail signaling pathway in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Kai Li,Li-Xuan Liu,Wei-Yi Zhang,Hao-Lian Zhan,Rui-Pei Chen,Jia-Lin Feng,Ling-Fei Wu

International journal of oncology 54:1665-1675 PubMed30816435

2019

lncRNA CASC9 positively regulates CHK1 to promote breast cancer cell proliferation and survival through sponging the miR‑195/497 cluster.

Applications

Unspecified application

Species

Unspecified reactive species

Guoli Shao,Mengchuan Wang,Xulong Fan,Lin Zhong,Zixiang Wang,Pusheng Zhang,Shufeng Ji

Analytical methods : advancing methods and applica 11:1027-1034 PubMed31656535

2019

A Nuclease Protection ELISA Assay for Colorimetric and Electrochemical Detection of Nucleic Acids.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica E Filer,Robert B Channon,Charles S Henry,Brian J Geiss

Current biology : CB 28:1137-1143.e5 PubMed29576476

2018

Unravelling the Molecular Determinants of Bee Sensitivity to Neonicotinoid Insecticides.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Manjon,Bartlomiej J Troczka,Marion Zaworra,Katherine Beadle,Emma Randall,Gillian Hertlein,Kumar Saurabh Singh,Christoph T Zimmer,Rafael A Homem,Bettina Lueke,Rebecca Reid,Laura Kor,Maxie Kohler,Jürgen Benting,Martin S Williamson,T G Emyr Davies,Linda M Field,Chris Bass,Ralf Nauen

Oncology letters 15:3621-3629 PubMed29467883

2018

Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wanqun Chen,Yaya Yu,Naikun Yang,Jingli Zhu,Ke Li,Ruocun Li,Wenqiao Su,Lina Luo,Ling Hu,Gengxin Chen,Haixia Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Digoxigenin antibody [HRP.21H8]

Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (AB6212)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (23)

Product promise