AB215342

HRP Anti-eIF2A antibody [EPR11042]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal EIF2A antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CDA02, MSTP004, MSTP089, EIF2A, Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A

2 Images

Lab

Western blot - HRP Anti-eIF2A antibody [EPR11042] (AB215342)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215342 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-eIF2A antibody [EPR11042] (ab215342) at 1/5000 dilution

Lane 1:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HAP1 WT at 20 µg

Lane 4:

eIF2A knockout HAP1 at 20 µg

Predicted band size: 65 kDa

Observed band size: 65 kDa

true

Exposure time: 8min

Lab

Western blot - HRP Anti-eIF2A antibody [EPR11042] (AB215342)

ab215342 was shown to specifically react with eIF2A in wild-type HAP1 cells as signal was lost in EIF2A (eIF2A) knockout cells. Wild-type and EIF2A (eIF2A) knockout samples were subjected to SDS-PAGE. ab215342 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-eIF2A antibody [EPR11042] (ab215342) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

EIF2A (eIF2A) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 65 kDa

Observed band size: 65 kDa

true

Exposure time: 8min

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-eIF2A antibody [EPR11042]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-eIF2A antibody [EPR11042]
Unconjugated

Anti-eIF2A antibody [EPR11042]
Carrier free

Anti-eIF2A antibody [EPR11042] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11042

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eIF2A also referred to as eukaryotic translation initiation factor 2A is a protein involved in the initiation of mRNA translation. The eIF2A protein is distinct from eIF2α despite the similarity in their names. Its molecular weight is approximately 65 kDa. Expression of eIF2A occurs mainly in the cytoplasm of cells where it participates in the protein synthesis process. eIF2A functions as a mediator that targets the initiator methionylated-tRNA to the small ribosomal subunit in a manner independent from eIF2 complex.

Biological function summary

EIF2A impacts the translation mechanism by facilitating the binding of Met-tRNAi to the ribosome under particular conditions especially during stress responses where canonical eIF2 might be inhibited. It operates independently from the larger eIF2 complex which also includes eIF2β and eIF2γ subunits. This independent activity allows eIF2A to serve as a backup mechanism for translation initiation when the normal pathway is impaired.

Pathways

Translation regulation involves interaction with cellular stress pathways and the integrated stress response (ISR). eIF2A operates within pathways that respond to physiological stresses such as nutrient deprivation and oxidative stress which trigger alterations in protein synthesis rates. eIF2A is related to the eIF2 kinase which phosphorylates the α subunit of eIF2 leading to a general reduction in translation initiation under stress allowing selective translation of stress-response proteins.

Irregularities in eIF2A function link to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like diabetes. These conditions often involve disruptions in the stress response pathways where eIF2A participates. The protein participates with others like PERK a kinase that phosphorylates eIF2α under endoplasmic reticulum stress contributing to disease-related changes in protein synthesis. By modulating translation under stress eIF2A becomes significant in these pathological contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.

See full target information EIF2A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 14:1138858 PubMed37388447

2023

Network pharmacology-based approach to explore the underlying mechanism of sinomenine on sepsis-induced myocardial injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Linggang Sun,Zhiyun Chen,Yunjie Ni,Zhengfei He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-eIF2A antibody [EPR11042]

Western blot - HRP Anti-eIF2A antibody [EPR11042] (AB215342)

Western blot - HRP Anti-eIF2A antibody [EPR11042] (AB215342)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise