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AB204386

HRP Anti-ELMO1 antibody [EPR12919]

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(1 Publication)

Rabbit Recombinant Monoclonal ELMO1 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0281, ELMO1, Engulfment and cell motility protein 1, Protein ced-12 homolog

1 Images
Western blot - HRP Anti-ELMO1 antibody [EPR12919] (AB204386)
  • WB

Lab

Western blot - HRP Anti-ELMO1 antibody [EPR12919] (AB204386)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204386 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ELMO1 antibody [EPR12919] (ab204386) at 1/5000 dilution

Lane 1:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Predicted band size: 84 kDa

Observed band size: 84 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12919

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ELMO1 also known as Engulfment and Cell Motility 1 protein functions in the regulation of cell migration and cytoskeletal reorganization. It possesses a molecular mass of approximately 82 kDa. This protein expresses across many tissues including the brain lung and kidney indicating widespread roles in different cellular processes. Researchers frequently study ELMO1 due to its involvement in essential cellular functions linking to motility and immune responses.
Biological function summary

The protein ELMO1 participates in the regulation of Rac signaling pathways through the formation of complexes with DOCK family proteins. This complex formation contributes to the activation of Rac a small GTPase critical for actin cytoskeleton dynamics. ELMO1 affects processes such as phagocytosis and cell migration by modulating cytoskeletal configurations showing its role in the cellular machinery controlling cell shape and movement.

Pathways

The ELMO1 protein contributes to the Rac1 signaling and the phagocytosis pathway. These pathways play essential roles in integrating extracellular signals to promote cytoskeletal rearrangement and cellular movement. ELMO1 interacts with proteins like DOCK180 to facilitate these pathways. Through these interactions ELMO1 orchestrates cellular responses including actin polymerization and membrane extensions enabling effective cellular reactions to external stimuli.

Research links ELMO1 to conditions such as diabetic nephropathy and inflammatory disorders. Elevated levels of ELMO1 expression correlate with increased diabetic nephropathy progression implicating its role in this disease. In inflammatory disorders ELMO1 coordinates with proteins like Rac1 to influence macrophage function and other immune cell behaviors. These interactions show ELMO1 as an important factor in disease mechanisms where cell migration and immune responses play a significant role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
See full target information ELMO1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34279220

2021

Ferroptotic stress promotes the accumulation of pro-inflammatory proximal tubular cells in maladaptive renal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shintaro Ide,Yoshihiko Kobayashi,Kana Ide,Sarah A Strausser,Koki Abe,Savannah Herbek,Lori L O'Brien,Steven D Crowley,Laura Barisoni,Aleksandra Tata,Purushothama Rao Tata,Tomokazu Souma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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