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AB306348

HRP Anti-Emerin antibody [EPR11071]

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Rabbit Recombinant Monoclonal Emerin antibody - conjugated to HRP.

View Alternative Names

EDMD, STA, EMD, Emerin

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11071

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Emerin also referred to as the emerin protein is a nuclear envelope protein with a mass of approximately 34 kDa. It belongs to a family of LEM domain proteins that localize at the inner nuclear membrane. Emerin predominantly expresses in cardiac and skeletal muscles but also appears in other tissues. Its primary mechanical role involves binding to the nuclear lamina structures and interacting with various nuclear proteins to maintain nuclear integrity and architecture.
Biological function summary

Emerin serves as an important component in mechanical signal transduction pathways. It interacts with barrier-to-autointegration factor (BAF) forming an important part of the nuclear envelope complex. This protein complex helps in chromatin organization and regulation of gene expression. Emerin influences nuclear assembly and shape and modulates chromatin attachment to the nuclear envelope playing an important role in nuclear processes.

Pathways

Emerin participates actively in the Emery-Dreifuss muscular dystrophy pathway and affects the interplay of other nuclear lamina components like lamin A/C. It stabilizes chromatin structure and cooperates with proteins such as nesprins and SUN proteins to regulate nuclear-cytoskeletal interactions. Emerin's involvement in these pathways highlights its role in maintaining mechanical resilience and functionality of the nuclear envelope impacting gene expression dynamics.

Emerin mutations relate to Emery-Dreifuss muscular dystrophy (EDMD) and other musculoskeletal disorders. Alteration in emerin disrupts its interaction with lamin A/C and BAF leading to nuclear envelope defects. This dysfunction manifests in muscle weakness and cardiac anomalies typical of EDMD. Understanding emerin's role offers insights into pathogenesis and potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non-farnesylated prelamin-A/C. Together with NEMP1, contributes to nuclear envelope stiffness in germ cells (PubMed : 32923640). EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
See full target information EMD
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